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Visar resultat 1 - 5 av 8 avhandlingar som matchar ovanstående sökkriterier.

1. 1. Methods for Generation and Characterization of Monospecific Antibodies

   Författare :Johan Rockberg; Mathias Uhlén; John McCafferty; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody; epitope mapping; HER2; human protein atlas; immunogenicity; proteomics; Bioengineering; Bioteknik;

   Sammanfattning : Recent advances in biotechnology have generated possibilities to investigate and measure parts of life previously left for believers to explain. Utilizing the same book of recipes, the genome, our cells produce selections of proteins at a time and thereby niche into a multitude of specialized cell types, tissues and organs comprising our body. LÄS MER

3. 2. Engineering enzymes towards biotherapeutic applications using ancestral sequence reconstruction

   Författare :Natalie Hendrikse; Per-Olof Syrén; Johan Rockberg; Erik Nordling; Reinhard Sterner; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; enzyme engineering; enzyme evolution; ancestral sequence reconstruction; therapeutic enzymes; terpene cyclases; ammonia-lyases; sulfatases; lysosomal enzymes; Kemi; Chemistry;

   Sammanfattning : Enzymes are versatile biocatalysts that fulfill essential functions in all forms of life and, therefore, play an important role in health and disease. One specific application of enzymes in life science is their use as biopharmaceuticals, which typically benefits from high catalytic activity and stability. LÄS MER

4. 3. Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization

   Författare :Francis Jingxin Hu; Johan Rockberg; John McCafferty; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody engineering; antibody affinity maturation; combinatorial protein engineering; epitope mapping; FACS; HER2; complement C5; Staphylococcal surface display; surface display.; Bioteknologi; Biotechnology;

   Sammanfattning : Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. LÄS MER

5. 4. Methods for cell line and protein engineering

   Författare :Magnus Lundqvist; Johan Rockberg; Mathias Uhlén; Diethard Mattanovich; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell line development; therapeutic proteins; protein engineering; molecular cloning; mutagenesis; split-GFP; Bioteknologi; Biotechnology;

   Sammanfattning : Therapeutic proteins are becoming increasingly important. They are desirable, as they typically possess low adverse effects and higher specificity compared to the traditional, small molecule drugs. But they are also more complex and involve different intricate and expensive development and production processes. LÄS MER

7. 5. Platforms and strategies harnessing signaling pathways of multifactorial diseases by multispecific antibodies

   Författare :Aman Mebrahtu; Johan Rockberg; Fredrik Y. Frejd; Janine Schuurman; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibodies; Bispecific antibodies; Multispecific affinity proteins; Protein engineering; Protein expression; Cancer; Immunotherapy; Autoimmunity; Systemic Lupus Erythematosus; Affibody molecules; AffiMab; Antikroppar; Bispecifika antikroppar; Multispecifika affinitets protein; Protein engineering; Protein uttryck; Cancer; Immunoterapai; Autoimmunitet; Systemic Lupus Erythematosus; Affibody molekyler; AffiMab; Biotechnology; Bioteknologi;

   Sammanfattning : Abstract [ENG]Proteins govern a multitude of biological functions vital to sustain life. The ability to withstand diseases development and harboring a defense against foreign pathogens is attributed to the wonders of the immune system and the proteins and cells its comprised of. LÄS MER