Sökning: "rockberg"

Visar resultat 1 - 5 av 7 avhandlingar innehållade ordet rockberg.

  1. 1. Methods for Generation and Characterization of Monospecific Antibodies

    Författare :Johan Rockberg; Mathias Uhlén; John McCafferty; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; antibody; epitope mapping; HER2; human protein atlas; immunogenicity; proteomics; Bioengineering; Bioteknik;

    Sammanfattning : Recent advances in biotechnology have generated possibilities to investigate and measure parts of life previously left for believers to explain. Utilizing the same book of recipes, the genome, our cells produce selections of proteins at a time and thereby niche into a multitude of specialized cell types, tissues and organs comprising our body. LÄS MER

  2. 2. Engineering enzymes towards biotherapeutic applications using ancestral sequence reconstruction

    Författare :Natalie Hendrikse; Per-Olof Syrén; Johan Rockberg; Erik Nordling; Reinhard Sterner; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; enzyme engineering; enzyme evolution; ancestral sequence reconstruction; therapeutic enzymes; terpene cyclases; ammonia-lyases; sulfatases; lysosomal enzymes; Kemi; Chemistry;

    Sammanfattning : Enzymes are versatile biocatalysts that fulfill essential functions in all forms of life and, therefore, play an important role in health and disease. One specific application of enzymes in life science is their use as biopharmaceuticals, which typically benefits from high catalytic activity and stability. LÄS MER

  3. 3. Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization

    Författare :Francis Jingxin Hu; Johan Rockberg; John McCafferty; KTH; []
    Nyckelord :ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody engineering; antibody affinity maturation; combinatorial protein engineering; epitope mapping; FACS; HER2; complement C5; Staphylococcal surface display; surface display.; Bioteknologi; Biotechnology;

    Sammanfattning : Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. LÄS MER

  4. 4. Methods for cell line and protein engineering

    Författare :Magnus Lundqvist; Johan Rockberg; Mathias Uhlén; Diethard Mattanovich; KTH; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell line development; therapeutic proteins; protein engineering; molecular cloning; mutagenesis; split-GFP; Bioteknologi; Biotechnology;

    Sammanfattning : Therapeutic proteins are becoming increasingly important. They are desirable, as they typically possess low adverse effects and higher specificity compared to the traditional, small molecule drugs. But they are also more complex and involve different intricate and expensive development and production processes. LÄS MER

  5. 5. Strategies to improve and balance the expression levels of recombinant proteins in mammalian cell lines

    Författare :Mona Moradi Barzadd; Johan Rockberg; Alan Dickson; KTH; []
    Nyckelord :MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; CHO; HEK293; Aggregation; Bispecific; Cancer assay; Cell line engineering; sulfatase; biologics; transcriptomic; Biotechnology; Bioteknologi;

    Sammanfattning : Proteins are the building blocks of all living organisms enabling us to function and survive. There are more than 100,000 different proteins in the human body performing a variety of vital tasks. LÄS MER