Sökning: "reverse genetics"
Visar resultat 1 - 5 av 37 avhandlingar innehållade orden reverse genetics.
1. HIV-1 reverse transcriptase activity assays based on monoclonal antibodies to native enzyme : Applications on studies of antiviral substances and immunological variation of reverse transcriptase
Sammanfattning : Specific detection of HIV-1 reverse transcriptase (RT) activity in crude materials was enhanced using a capture assay for immunopurification and subsequent activity assay. The purification step was achieved by anti-HIV-1 RT monoclonal antibodies (Mabs) immobilized onto a solid carrier. LÄS MER
2. Repetitive DNA in search of a function - a study of telomeric and centromeric sequences in Chironomus
Sammanfattning : Repetitive DNA is quantitatively the main component of telomeres and centromeres, structures responsible for maintenance of the eukaryotic chromosome. The telomere is the specialized nucleoprotein complex that terminates linear chromosomes. LÄS MER
3. Telomeric DNA in Chironomus, a naturally telomerase-free system
Sammanfattning : Telomeres are specialized complexes of DNA and associated proteins that protect the ends of eukaryotic chromosomes. Although most organisms have short repeated DNA sequences at their telomeres, which are specified and maintained by the enzyme telomerase, there are a few exceptions. LÄS MER
4. Expression profiling using manifold supports
Sammanfattning : Analyses of mRNA provides a condensed view of gene structure and quantitative analysis can reveal the induction of physiological or pathological gene expression programs. This thesis describes a new method for mRNA isolation, followed by sensitive real time detection via polymerase chain reaction (PCR), in order to quantitate transcripts of interest. LÄS MER
5. Genetic Characterization of Bone and Soft Tissue Tumors
Sammanfattning : Bone and soft tissue tumors (BSTT) constitute a heterogeneous group of neoplasms of mesenchymal and neuroectodermal origin. Although many BSTT are rare, it has become clear that BSTT are characterized by recurrent acquired chromosomal aberrations, and the general aim of this thesis have been to apply molecular genetic and molecular cytogenetic techniques to further characterize recurrent breakpoints and deletions, and to search for candidate target genes. LÄS MER