Sökning: "reporter assay"

Visar resultat 1 - 5 av 74 avhandlingar innehållade orden reporter assay.

  1. 1. Development of sensitive cellular assay systems and their application to the identification of "orphan" seven-transmembrane receptors

    Författare :Knut Kotarsky; Slemhinnans immunologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biokemi; Metabolism; Biochemistry; metabolism; leukotrieneB4; thiazolidinedione; 9-HODE; fatty acid; luciferase; GFP; reporter gene; preclinical assay; receptor; GPCR;

    Sammanfattning : Seven-transmembrane, G-protein coupled receptors play a central role in physiology since they facilitate cell communication in multicellular organisms by recognition of a broad range of ligands. They also represent important drug targets. LÄS MER

  3. 2. Effects of galectins and antibodies in HIV infection; Novel Assays

    Författare :Enas Sheik-Khalil; Institutionen för laboratoriemedicin; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; HIV-1; galectin-1; galectin-8; neutralizing antibodies; cell-cell fusion; Microscale anisotropy assay; virus binding assay; glycosylation; gp120; Automated plaque reduction assay; CellProfiler; NeutNet;

    Sammanfattning : The high variability of the HIV envelope glycoproteins (Env), and their heavy glycan coating, contributes to the limited host immune control. Still, broadly neutralizing antibodies (bnAbs) are found in some chronically infected HIV-infected individuals, which has spurred the research on antibody-based vaccines. LÄS MER

  4. 3. Proximity Ligation and Barcoding Assays : Tools for analysis of proteins and protein complexes

    Författare :Di Wu; Masood Kamali-Moghaddam; Ulf Landegren; Ola Söderberg; Jörg Hoheisel; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proximity ligation assay; proximity barcoding assay; sensitive; in situ; multiplex; profiling; amyloid-beta; MARK; tau; CSF; biomarker; protein complexes; Alzheimer’s disease; amyotrophic lateral sclerosis; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Proteins are fundamental structural, enzymatic and regulatory components of cells. Analysis of proteins, such as by measuring their concentrations, characterizing their modifications, and detecting their interactions, provides insights in how biological systems work physiologically or pathologically at the molecular level. LÄS MER

  5. 4. Identification and evaluation of antiviral compounds targeting Rift Valley fever virus

    Författare :Koushikul Islam; Clas Ahlm; Magnus Evander; Anna K. Överby; Johan Neyts; Umeå universitet; []
    Nyckelord :Rift Valley fever virus; antiviral; assay development; screening; drug discovery;

    Sammanfattning : Rift Valley fever virus (RVFV), a negative-stranded RNA virus, is the etiological agent of the vector-borne zoonotic disease Rift Valley fever (RVF). RVFV causes significant morbidity and mortality in humans and livestock throughout Africa and the Arabian Peninsula. LÄS MER

  7. 5. Identification of novel inducers for LL-37 expression : assay development and mechanistic studies

    Författare :Frank Nylén; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The remaining reservoir of effective antibiotics is running out and more and more pathogens are gaining resistance, even to the last line of antibiotics. Therefore, novel treatment regimens against bacterial infections are urgently needed. LÄS MER