Sökning: "recombinant surface proteins"

Visar resultat 1 - 5 av 96 avhandlingar innehållade orden recombinant surface proteins.

  1. 1. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

    Författare :Emma Bäcklund; Gen Larsson; Olle Holst; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; Biology; Biologi;

    Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER

  3. 2. Metal Binding Tags - Characterisation, Use in Bioseparation and Applications of Green Fluorescent Fusion Proteins

    Författare :Florent Bernaudat; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteiner; enzymologi; enzymology; E. coli; Immobilised metal ion affinity chromatography; Affinity tags; Green fluorescent protein; Surface plasmon resonance; Genetic engineering; Screening; Proteins; Immunoassay; Biotechnology; Bioteknik;

    Sammanfattning : Most of the recombinant proteins produced nowadays are fused to affinity tags, in order to facilitate their purification through affinity chromato-graphy. Out the different tags available, poly-histidine tags are among the most commonly used and can help purification through immobilised metal ion affinity chromatography (IMAC). LÄS MER

  4. 3. Recombinant subunit vaccines : protein immunogens

    Författare :Sissela Liljeqvist; KTH; []
    Nyckelord :;

    Sammanfattning : Recombinant techniques provide valuable tools for thedevelopment of modern subunit vaccines. In this thesisdifferent systems for production of recombinant subunitvaccines are presented. The strategies investigated includeprotein immunogens, live bacterial vectors and nucleicacids. LÄS MER

  5. 4. Hierarchical Assembly Investigations and Multiscale Characterization of Protein-based Materials : Insights from Whey Protein Nanofibrils and Recombinant Spider Silk Microspheres

    Författare :Eirini Ornithopoulou; My Hedhammar; Thomas Crouzier; Christina Divne; Tim Melander Bowden; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Hierarchical Assembly; Protein-based materials; Whey Protein Nanofibrils; β-Lactoglobulin; Recombinant Spider Silk; Microspheres; Biomedical Applications; Surface Biofunctionalization; Hierarkisk montering; Proteinbaserade material; Vassleprotein nanofibriller; β-Laktoglobulin; Rekombinant spindelsilke Mikrosfärer; Biomedicinska tillämpningar; Ytbiokonjugering; Biotechnology; Bioteknologi;

    Sammanfattning : Protein-based materials, with their unique properties of combining high strength, while maintaining elasticity, and their inherent biocompatibility, hold immense potential for various applications. In order to harness these properties, we need to understand and control how protein building blocks come together to form hierarchically structured materials. LÄS MER

  7. 5. Streptococcal cell surface proteins : structure and gene characterisation

    Författare :Viatcheslav Katerov; Avdelningen för medicinsk mikrobiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Mikrobiologi; mycology; virology; bacteriology; virologi; mykologi; Microbiology; bakteriologi;

    Sammanfattning : Streptococcus pyogenes (group A streptococci, GAS) is a potent human pathogen known to express a number of cell surface associated virulence factors. It is an established fact that these different surface proteins play an important role during infection. LÄS MER