Sökning: "recombinant selenoprotein production"

Hittade 5 avhandlingar innehållade orden recombinant selenoprotein production.

  1. 1. Stretching the genetic code : incorporation of selenocysteine at specific UGA codons in recombinant proteins produced in Escherichia coli

    Författare :Olle Rengby; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Selenocysteine (Sec) exists in all domains of life and represents the 21st naturally occurring amino acid. A Sec residue is co-translationally incorporated at a predefined opal (UGA) codon. UGA codons normally encode for translational stop via the protein release factor 2 (RF2). LÄS MER

  3. 2. Selenocysteine in proteins : properties and biotechnological use

    Författare :Linda Johansson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Selenocysteine; recombinant selenoprotein production; thioredoxin reductase; enzymology; protein labeling; mite allergy; mouse model;

    Sammanfattning : Selenocysteine (Sec), the 21st amino acid, exists in all kingdoms of life and has unique biochemical properties, such as high electrophilicity and low pKa. The highly increased reactivity of selenoenzymes compared to their sulfur-containing cysteine-dependent homologs is generally regarded as the evolutionary reason for having selenoproteins. LÄS MER

  4. 3. Development of a Sel-tag for multimodality imaging and studies of mammalian thioredoxin reductase 1

    Författare :Qing Cheng; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Selenocysteine (Sec; U in one-letter code), the 21st amino acid existing in all selenoproteins, has unique biochemical properties due to its selenium atom, including a low pKa and a high reactivity with many electrophilic agents. When a selenoprotein is translated, the insertion of Sec occurs at a UGA codon, normally yielding translational termination. LÄS MER

  5. 4. Mammalian thioredoxin reductase 1 in antioxidant defense, regulation of adipocyte differentiation and as an anticancer drug target

    Författare :Xiaoxiao Peng; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Reactive oxygen species (ROS) are oxygen containing reactive molecules generated as by-products of cellular metabolism. At physiological concentrations, ROS acts as secondary messengers in cellular signaling transduction, but excessive amounts of ROS result in oxidative stress and cellular damage. LÄS MER

  7. 5. Targeting glutathione peroxidases : identifying environmental modulators, and screening for novel small molecule inhibitors

    Författare :Dorian M. Cheff; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Glutathione peroxidases (GPXs) are a family of selenoproteins that are critical regulators of reactive oxygen species (ROS) in the cell, specifically hydroperoxides like hydrogen peroxide (H2O2). ROS are important for normal cell signaling and are tightly controlled to promote cell growth, proliferation, and survival. LÄS MER