Sökning: "recombinant proteins"

Visar resultat 16 - 20 av 374 avhandlingar innehållade orden recombinant proteins.

1. 16. Methodology for high-throughput production of soluble recombinant proteins in Escherichia coli

   Författare :Katrin Markland; Gen Larsson; Pär Nordlund; Jenny Ottosson; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; high-throughput methodology; recombinant protein production; solubility; productivity; quality; cultivation technology; parallel reactors; Biochemistry; Biokemi;

   Sammanfattning : The aim of this work was to investigate and determine central parameters that can be used to control and increase the solubility, quality and productivity of recombinant proteins. These central parameters should be applicable under the constraints of high-throughput protein production in Escherichia coli. LÄS MER

3. 17. Recombinant amelogenin - Strategies for purification and protein processing

   Författare :Johan Svensson Bonde; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Recombinant amelogenin; protein purification; fusion protein; purification tag; enamel; hydroxyapatite; matrix protein; dental regeneration; wound healing; bone formation;

   Sammanfattning : Amelogenin is an extracellular matrix protein involved in formation of enamel during early tooth development. By interacting with the forming enamel crystallites amelogenin affects their growth, so that highly elongated hydroxyapatite crystals are formed. LÄS MER

4. 18. Production and folding of proteins in the periplasm of Escherichia coli

   Författare :Rageia Elfageih; Jan-Willem de Gier; Hans-Georg Koch; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; periplasm; recombinant protein production; disulfide bond containing proteins; translation initiation region; protein translocation machinery; co-translational folding; neuraminidases; biokemi; Biochemistry;

   Sammanfattning : The Gram-negative bacterium E. coli is the most widely used host for the production of recombinant proteins. Disulfide bond containing recombinant proteins are usually produced in the periplasm of E. coli since in this compartment of the cell - in contrast to the cytoplasm - disulfide bond formation is promoted. LÄS MER

5. 19. Functional studies on the Light-harvesting-Like (LiL) Proteins in Cyanobacteria and Cryptophytes

   Författare :Tania Tibiletti; Christiane Funk; Martin Hagemann; Umeå universitet; []
   Nyckelord :Photosynthesis; cyanobacteria; Guillardia theta; photosystem II; chlorophyll-binding proteins; onehelix LiL proteins; photoprotection; biokemi; Biochemistry;

   Sammanfattning : The light-harvesting like (LiL) proteins are a widely spread group of proteins within photosynthetic organisms. They are membrane proteins composed of one to four transmembrane helices and – in homology to the light-harvesting complexes of algae and higher plants – at least one of these transmembrane helices contains the chlorophyll a/b-binding (CAB) domain. LÄS MER

7. 20. Recombinant Antibodies – Tools for building proteomic platforms

   Författare :Anna Säll; Institutionen för immunteknologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody development; recombinant scFv antibodies; phage display; affinity proteomics; mass spectrometry;

   Sammanfattning : This thesis, which is based on four original papers, covers different aspects of the evolvement and development of proteomic platforms and the construction of human synthetic antibody libraries for the isolation of antibody reagents has been very central. There is an accelerating need for technologies capable of providing proteomic analysis of high sensitivity with good accuracy and an overall broad coverage as well as for biomarker validation. LÄS MER