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  1. 1. Impact of glucose uptake rate on recombinant protein production in Escherichia coli

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Emma Bäcklund; KTH.; [2011]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; AIDA-autotransporter; Escherichia coli; fed-batch; glucose uptake rate; integral membrane proteins; outer membrane proteins; periplasmic retention; phosphotransferase system; recombinant proteins; specific growth rate; surface expression; NATURAL SCIENCES Biology; NATURVETENSKAP Biologi;

    Sammanfattning : Escherichia coli (E. coli) is an attractive host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve high product quantities. LÄS MER

  2. 2. Protein production in the E. coli cell envelope

    Detta är en avhandling från Stockholm : Department of Biochemistry and Biophysics, Stockholm University

    Författare :Thomas Baumgarten; Stockholms universitet.; [2018]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; E. coli; protein biogenesis; recombinant proteins; membrane proteins; secretory proteins; protein displays; outer membrane vesicles; biokemi; Biochemistry;

    Sammanfattning : Proteins fulfil essential functions in every cell and malfunctioning proteins are often the cause of diseases. On the other hand, proteins like antibody fragments or hormones can be used to treat diseases. LÄS MER

  3. 3. Integration of Aqueous Two-Phase Systems into Recovery Processes for Biomolecules

    Detta är en avhandling från Folke Tjerneld, Department of Biochemistry, Lund University

    Författare :Cecilia Kepka; Lund University.; Lunds universitet.; [2004]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteins; proteins; plasmid DNA; large-scale; protein purification; integration; Expression; aqueous two-phase systems; enzymology; Proteiner; enzymologi;

    Sammanfattning : The main scope of this thesis has been to study and develop new purification methods and also to further develop existing methods for purification to enhance their efficacy. Important parameters in the development of the methods are that they should be suitable for scale-up and possible to be integrated into recovery processes. LÄS MER

  4. 4. Expression and purification of membrane proteins: Focus on the G-protein coupled receptor MC4r

    Detta är en avhandling från Viveka Dolby, Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, S-221 00 Lund

    Författare :Viveka Dolby; Lund University.; Lunds universitet.; [2004]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; enzymology; Proteiner; enzymologi; Proteins; melanocortin 4 receptor; GPCR; purification; Expression; membrane proteins;

    Sammanfattning : Membrane proteins are crucial components of the cell and are involved in many biological processes. Recombinant overexpression systems together with different purification methods are necessary to obtain large amounts of purified receptor for biophysical and functional studies. LÄS MER

  5. 5. Human antibody responses to hantavirus recombinant proteins & development of diagnostic methods

    Detta är en avhandling från Umeå : Umeå universitet

    Författare :Fredrik Elgh; Umeå universitet.; [1996]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Puumala virus; hantavirus infections; recombinant proteins; capsid; humoral immunity; immunoassay;

    Sammanfattning : Rodent-borne hantaviruses (family Bunyaviridae) cause two distinct human infections; hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). HFRS is a common viral zoonosis, characterized by fever, renal dysfunction and hemostatic imbalance. LÄS MER