Sökning: "recombinant protein"

Visar resultat 6 - 10 av 501 avhandlingar innehållade orden recombinant protein.

  1. 6. Human Aquaporins: Production, Characterization and Interactions

    Författare :Jennie Sjöhamn; Göteborgs universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; aquaporins; membrane protein; protein production; protein characterization; protein:protein interactions; structural biochemistry;

    Sammanfattning : Membrane proteins are essential components of the cell and responsible for the communication with the outside environment and transport of molecules across the membrane. Water transport is facilitated by aquaporins, which are water selective transmembrane pores that serve to maintain cell homeostasis. LÄS MER

  3. 7. Enhancing membrane and secretory protein production yields in Escherichia coli

    Författare :Zhe Zhang; Jan-Willem de Gier; Claes von Wachenfeldt; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; recombinant protein production; Escherichia coli; membrane protein; secretory protein; strain engineering; Biochemistry; biokemi;

    Sammanfattning : Proteins fulfill essential functions in living cells. To produce sufficient amounts of a protein is essential to study the structure and function of a protein, or to use it for medical purposes. Escherichia coli is a Gram-negative bacterium that is widely used for recombinant protein production. LÄS MER

  4. 8. Protein production in the E. coli cell envelope

    Författare :Thomas Baumgarten; Jan-Willem de Gier; Dirk-Jan Scheffers; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; E. coli; protein biogenesis; recombinant proteins; membrane proteins; secretory proteins; protein displays; outer membrane vesicles; Biochemistry; biokemi;

    Sammanfattning : Proteins fulfil essential functions in every cell and malfunctioning proteins are often the cause of diseases. On the other hand, proteins like antibody fragments or hormones can be used to treat diseases. LÄS MER

  5. 9. Metabolic Engineering of Recombinant Protein Production by Saccharomyces cerevisiae

    Författare :Zihe Liu; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; recombinant protein production; genome sequencing; DNA microarray; insulin precursor; growth rate; UV mutation; yeast; anaerobic electron acceptor; secretory pathway; unfolded protein response; metabolic engineering; systems biology; α-amylase;

    Sammanfattning : The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and it also provides a platform for the production of many heterologous proteins of medical or industrial interest. In this thesis, random and rational approaches, such as vector design, host engineering, fermentation analysis, UV Mutation, coupled with high-throughput systems biology techniques (including whole genomic sequencing, microarray analysis and flux analysis) and integrated analysis (Reporter feature technique), were employed to engineer cellular properties more effectively and purposefully to construct cell factories for protein production. LÄS MER

  7. 10. Control of Proteolysis of Recombinant Proteins in Escherichia coli

    Författare :Aleksei Rozkov; KTH; []
    Nyckelord :proteolysis; recombinant protein; E. coli; bioprocess scale-up; protein A; ATP; sulfite; amino acids; growth inhibition; fed-batch culture; ZZT2;

    Sammanfattning : .... LÄS MER