Sökning: "recombinant protein"

Visar resultat 11 - 15 av 501 avhandlingar innehållade orden recombinant protein.

1. 11. Proximity Ligation Assay for High Performance Protein Analysis in Medicine

   Författare :Gucci Jijuan Gu; Ulf Landegren; Thomas Joos; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein; post-translational modification; interactions; protein domain; western blotting; MARK; tau; AD; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

   Sammanfattning : High quality reagents are preconditions for high performance protein analyses. But despite progress in some techniques, e.g. mass spectrometry, there is still a lack of affinity-based detection techniques with enhanced precision, specificity, and sensitivity. LÄS MER

3. 12. Membrane and secretory protein production in Escherichia coli

   Författare :Alexandros Karyolaimos; Jan-Willem de Gier; Matthew DeLisa; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Escherichia coli; recombinant protein; tunable protein production; cytoplasmic membrane; periplasm; signal peptide; force profile analysis; co-translational folding; Biochemistry; biokemi;

   Sammanfattning : Escherichia coli (E. coli) is the most widely used bacterium for the production of recombinant proteins. However, the production of proteins in its cytoplasmic membrane or periplasm is challenging. Therefore, the aim of this doctoral thesis was to develop setups for enhancing the production of membrane and secretory proteins in E. LÄS MER

4. 13. Recombinant Enzymes in Pyrosequencing Technology

   Författare :Nader Nourizad; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

   Sammanfattning : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. LÄS MER

5. 14. Bioprocess Monitoring and Control in Pseudomonas cepacia and recombinant Escherichia coli cultivations

   Författare :Anita Tocaj; LTH Ingenjörshögskolan vid Campus Helsingborg; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein L; expanded bed; flow-injection analysis; recombinant protein production; acetic acid; on-line measurements; Pseudomonas cepacia; recombinant Escherichia coli; Fed-batch cultivations; control strategies; xylanase deletion derivatives from Rhodothermus marinus; Biotechnology; Bioteknik;

   Sammanfattning : The control of cultivation conditions and its influence on growth and production have been investigated. The use of fed-batch processes have been demonstrated for several cultivation systems and so has the use of different control signals in these systems. LÄS MER

7. 15. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

   Författare :My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

   Sammanfattning : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. LÄS MER