Sökning: "recombinant libraries"

Visar resultat 1 - 5 av 25 avhandlingar innehållade orden recombinant libraries.

1. 1. Directed Enzyme Evolution of Theta Class Glutathione Transferase : Studies of Recombinant Libraries and Enhancement of Activity toward the Anticancer Drug 1,3-bis(2-Chloroethyl)-1-nitrosourea

   Författare :Anna-Karin Larsson; Bengt Mannervik; Ian Cotgreave; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; glutathione transferase; directed evolution; 1; 3-bis 2-chloroethyl -1-nitrosourea; alkyltransferase; recombinant libraries; chloroethylnitrosourea; multi-dimensional data analysis; Biokemi; Biochemistry; Biokemi;

   Sammanfattning : Glutathione transferases (GSTs) are detoxication enzymes involved in the cellular protection against a wide range of reactive substances. The role of GSTs is to catalyze the conjugation of glutathione with electrophilic compounds, which generally results in less toxic products. LÄS MER

3. 2. Recombinant Antibodies – Tools for building proteomic platforms

   Författare :Anna Säll; Institutionen för immunteknologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody development; recombinant scFv antibodies; phage display; affinity proteomics; mass spectrometry;

   Sammanfattning : This thesis, which is based on four original papers, covers different aspects of the evolvement and development of proteomic platforms and the construction of human synthetic antibody libraries for the isolation of antibody reagents has been very central. There is an accelerating need for technologies capable of providing proteomic analysis of high sensitivity with good accuracy and an overall broad coverage as well as for biomarker validation. LÄS MER

4. 3. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

   Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

   Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER

5. 4. Biocatalytic transamination with recombinant Saccharomyces cerevisiae: Challenges and opportunities

   Författare :Nora Weber; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Saccharomyces cerevisiae; transamination; whole-cell biocatalysis; pyruvate; PLP; thiamine; reduction;

   Sammanfattning : Chiral building blocks are important molecules for synthesis of fine chemicals and pharmaceuticals. Biocatalysis has gained in relevance over traditional organic methods for synthesis of chiral compounds, as reactions can be performed at high enantio-selectivity and purity with environmentally friendly, simple, and cheap methods. LÄS MER

7. 5. Molecular Recognition in Antibody Engineering. Studies on recombinant and phage displayed antibodies

   Författare :Ann-Christin Malmborg Hager; Institutionen för immunteknologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; transplantation; affinity; antibody; kinetic; Immunologi; serologi; serology; Immunology; Bioteknik; Biotechnology; library; selection; surface display; phage display; association rate; dissociation rate;

   Sammanfattning : In vitro generation of antibody fragments of desired specificity and affinity plays an important role since it permits the generation of reagents which may be valuable for diagnostic and therapeutic applications. Furthermore, the kinetic parameters for an antibody-antigen interaction, rather than the affinity, has shown to correlate with biological functions like virus neutralization. LÄS MER