Sökning: "real-time quantitative RT-PCR"

Visar resultat 1 - 5 av 30 avhandlingar innehållade orden real-time quantitative RT-PCR.

  1. 1. The Identification and Characterisation of LRIG Gene Family and Its Expression in Astrocytic Tumours

    Författare :Dongsheng Guo; Tomas Ekström; Umeå universitet; []
    Nyckelord :astrocytoma; brain; EGFR; LRIG; leucine-rich repeat; real-time RT-PCR; onkologi; Oncology;

    Sammanfattning : Gliomas are the most common primary brain tumours, and their capacity to invade surrounding normal brain prevents complete removal of the tumour. Malignant glioma has still a poor prognosis. However, with the rapid development of molecular biology our understanding about glioma has increased dramatically. LÄS MER

  3. 2. Prognostic factors in colorectal cancer : aspects of tumour dissemination

    Författare :Åke Öberg; Gudrun Lindmark; Bengt Jeppsson; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Colorectal neoplasms; prognosis; lymph node metastases; disseminated tumour cells; cytokeratins; micrometastases; metalloproteinases; immune cells; real-time quantitative RT-PCR; carsinoembryonic antigen;

    Sammanfattning : .... LÄS MER

  4. 3. Methodological studies of eicosanoid enzyme gene expression in vascular endothelium exposed to biomechanical stress

    Författare :Roya Doroudi; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; prostanoids; endothelium; quantitative RT-PCR; perfusion model; shear stress; intraluminal pressure; immediate-early genes;

    Sammanfattning : Prostaglandins are the major eicosanoids synthesized by vascular endothelial cells, and the vasculature is an important target organ for their bioactive metabolites. In vitro studies have shown that biomechanical forces are important regulators of vascular expression of prostanoid enzymes and release of metabolites. LÄS MER

  5. 4. Improved methodologies for molecular detection and quantification of viruses in food

    Författare :Sofia Persson; Patrik Ellström; Magnus Simonsson; Wim van der Poel; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; foodborne virus; digital PCR; real-time PCR; validation; assay design; food safety; Medicinsk vetenskap; Medical Science;

    Sammanfattning : Foodborne viruses such as norovirus, hepatitis A virus and hepatitis E virus cause a high burden of disease worldwide. Reverse transcription (RT) quantitative real-time PCR (qPCR) is the current standard method for monitoring viral contamination in the food chain. LÄS MER

  7. 5. Minimal Residual Disease Assessment in Childhood Acute Lymphoblastic Leukemia

    Författare :Ingrid Thörn; Christer Sundström; Richard Rosenquist; Gisela Barbany; Johan Botling; Kjeld Schmiegelow; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Childhood acute lymphoblastic leukemia; minimal residual disease; IG TCR gene rearrangements; BCR-ABL1 fusion gene transcripts; real-time quantitative PCR; multicolor flow cytometry; Pathology; Patologi; Pathology; patologi;

    Sammanfattning : Traditionally, response to treatment in hematological malignancies is evaluated by light microscopy of bone marrow (BM) smears, but due to more effective therapies more sensitive methods are needed. Today, detection of minimal residual disease (MRD) using immunological and molecular techniques can be 100 times more sensitive than morphology. LÄS MER