Sökning: "real-time quantitative PCR"

Visar resultat 1 - 5 av 94 avhandlingar innehållade orden real-time quantitative PCR.

  1. 1. Kinetics Quality Assessment for real-time PCR

    Författare :Tzachi Bar; [2005]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; kinetics quality assessment; PCR quality assessment; outlier detection; real-time PCR;

    Sammanfattning : For proper DNA quantification by real-time PCR, compared samples should have similar PCR efficiencies. This assumption might not be always correct, as suggested by hundreds of publications dealing with PCR inhibition. LÄS MER

  2. 2. Pre-PCR Processing Strategies for Quantitative Detection of Food-Borne Pathogens using Real-Time PCR

    Detta är en avhandling från Department of Applied Microbiology, Lund University

    Författare :Petra Wolffs; Lunds universitet.; Lund University.; [2003]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; DNA polymerase; sample treatment; pre-PCR processing; real-time PCR; Food-borne pathogens;

    Sammanfattning : Since the development of real-time PCR, the technology has been widely applied in the field of diagnostics. In comparison with conventional PCR it has opened up the possibility of accurate quantification of microorganisms in clinical, environmental and food samples. LÄS MER

  3. 3. Development of Real-Time PCR Based Methods for Detection of Viruses and Virus Antibodies

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Amal Elfaitouri; Uppsala universitet.; [2006]
    Nyckelord :Microbiology; Real-time PCR; broadly targeted PCR; degenerated PCR; rTth DNA polymerase; thermolabile UNG; QPIA; IgM; Mikrobiologi;

    Sammanfattning : Quantitative real-time PCR (QPCR) technology has been very useful for diagnosis of viral diseases. QPCR has recently reached a level of sensitivity, simplicity, and reproducibility which allows a large number of samples to be screened rapidly, make it a suitable tool for the clinical virology diagnostics. LÄS MER

  4. 4. Diagnostic PCR for the Detection of Yersinia enterocolitica and Salmonella in the Food-Chain: Reliability of PCR Performance

    Detta är en avhandling från Applied Microbiology (LTH)

    Författare :Rickard Knutsson; Lunds universitet.; Lund University.; [2001]
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Livsmedelsteknik; Food and drink technology; Kemiteknik och kemisk teknologi; Chemical technology and engineering; real-time PCR; YPCE medium; Enrichment PCR; DNA polymerases; PCR inhibitors; pre-PCR processing; Salmonella; Diagnostic PCR; Yersinia enterocolitica; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : This thesis deals with the methodological advances of diagnostic PCR including reliability of PCR and pre-PCR processing of food and feed samples. Diagnostic PCR has been greatly improved by the introduction of the second generation of PCR, so-called real-time PCR. LÄS MER

  5. 5. Acute gastroenteritis in Rwandan children under five years of age investigated by real-time PCR

    Detta är en avhandling från Applied Microbiology (LTH)

    Författare :Jean-Claude Kabayiza; Göteborgs universitet.; Gothenburg University.; [2014]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Gastroenteritis; Real-time PCR;

    Sammanfattning : Acute gastroenteritis is a major cause of illness and death among children in developing countries. Knowledge about the aetiology is important to make the right priorities regarding preventive measures, and for the recommendation to use or not use antibiotics. LÄS MER