Sökning: "real-time PCR"

Visar resultat 16 - 20 av 296 avhandlingar innehållade orden real-time PCR.

1. 16. Improved methodologies for molecular detection and quantification of viruses in food

   Författare :Sofia Persson; Patrik Ellström; Magnus Simonsson; Wim van der Poel; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; foodborne virus; digital PCR; real-time PCR; validation; assay design; food safety; Medicinsk vetenskap; Medical Science;

   Sammanfattning : Foodborne viruses such as norovirus, hepatitis A virus and hepatitis E virus cause a high burden of disease worldwide. Reverse transcription (RT) quantitative real-time PCR (qPCR) is the current standard method for monitoring viral contamination in the food chain. LÄS MER

3. 17. On the Diagnosis and Management of Viral Respiratory Infections

   Författare :Robin Brittain-Long; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Respiratory virus; Respiratory tract infection; Real-time PCR; Multiplex PCR; Antibiotic use;

   Sammanfattning : Acute respiratory tract infections (ARTIs), are the most common infections in man, and represent a major global health burden. Viruses, most often causing a mild and self-limiting disease, yet with substantial morbidity and high costs for society, mainly cause upper respiratory tract infections. LÄS MER

4. 18. Real-time PCR for diagnosis of botulism and quantification of neurotoxin gene expression in Clostridium botulinum

   Författare :Ingrid Artin; Avdelningen för medicinsk mikrobiologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; detection; qRT-PCR; Clostridium botulinum; molecular methods;

   Sammanfattning : The objectives of the work presented in this thesis have been to develop molecular methods for (i) detection of Clostridium botulinum in food and clinical samples, and (ii) monitoring the expression of the neurotoxin gene, cnt, during different growth phases and under different growth conditions. For the diagnosis of botulism, a real-time PCR-based method including an internal amplification control for C. LÄS MER

5. 19. Monitoring important soil-borne plant pathogens in Swedish crop production using real-time PCR

   Författare :Charlotta Almquist; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES;

   Sammanfattning : The global demand for food will increase considerably in the nearest future and among the major constraints to agricultural productivity are biotic stresses caused by microorganisms. In this thesis, the causal agents of four of the most important soil-borne diseases threatening the Swedish production of oilseed rape, sugar beets and red clover were selected as targets for developing diagnostic assays using real-time PCR. LÄS MER

7. 20. Detection and Characterisation of Salmonella in Animal Feed Samples by PCR-Based Methods

   Författare :Charlotta Löfström; Teknisk mikrobiologi; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Food and drink technology; Livsmedelsteknik; Biotechnology; Bioteknik; Biokemisk teknik; Biochemical technology; Analytisk kemi; Analytical chemistry; mykologi; virologi; bakteriologi; Mikrobiologi; validation; Microbiology; bacteriology; virology; mycology; Salmonella; ribotyping; detection; animal feed; randomly amplified polymorphic DNA; pulsed-field gel electrophorsis; pre-PCR processing; PCR; polymerase chain reaction; genotyping;

   Sammanfattning : Animal feed is a recognised source of Salmonella enterica for farm livestock and may also indirectly cause infection in people consuming foods of animal origin. It is therefore important to have rapid, reproducible and specific methods for the detection of Salmonella in feed, and for the characterisation of strains for further epidemiological investigations or to trace the source of contamination in a production facility. LÄS MER