Sökning: "quantitative real-time reverse transcriptase PCR"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden quantitative real-time reverse transcriptase PCR.

1. 1. Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays

   Författare :Shaman Muradrasoli; Jonas Blomberg; Göran Friman; Maria Brytting; Uppsala universitet; []
   Nyckelord :Virology; Diagnosis of Viral RNA; QPCR; RNA virus; broadly targeted PCR; probe design strategy; Infectious diseases; Medicin; Medicine;

   Sammanfattning : As the area of nucleic acid based technologies develops, so will our understanding of how structural variations in DNA and RNA pathogens are associated with disease. The overall goal of this thesis is the development of broadly targeted measurement techniques for variable viral RNA by Real-Time PCR (here referred to as quantitative reverse transcriptase PCR, QRT-PCR). LÄS MER

3. 2. Regulation of vascular function by fluid mechanical forces. Development of a new vascular experimental platform for integrative physiological and molecular biological studies of living conduit vessels

   Författare :Li-Ming Gan; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; perfusion model; endothelium; smooth muscle cells; shear stress; pressure; nitric oxide; endothelin-1; vascular endothelial growth factor; c-jun; c-fos; real-time PCR; NO probe.;

   Sammanfattning : The vascular endothelium is a multifunctional interface between blood flow and target organs and is continuously exposed to fluid mechanical forces such as shear stress, compressive, and tensile forces. Both in vivo and in vitro data indicate that biomechanical forces exert important modulating effects on key vascular functions. LÄS MER

4. 3. Plasma Membrane Proteomics and Functional Characterization & Expression Profiling of Plant Aquaporins

   Författare :Erik Alexandersson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Växtbiokemi; Plant biochemistry; phosphorylation; barley; GFP; GUS; quantitative real-time reverse transcriptase PCR; microarray; aquaporin; MIP; Arabidopsis; plasma membrane; proteomics;

   Sammanfattning : In order to identify integral and peripheral proteins associated with the plasma membrane (PM), highly purified Arabidopsis PMs were analyzed by mass spectrometry. In total, 238 putative PM proteins were identified, of which 114 were predicted to have transmembrane domains or to be PM-anchored. LÄS MER

5. 4. Quantitative analysis of melanoma transcripts : with emphasis on methodological and biological variation

   Författare :Malin Farnebäck; Håkan Hedman; Linköpings universitet; []
   Nyckelord :MEDICINE; MEDICIN;

   Sammanfattning : The introduction of RT-PCR made it possible to detect circulating tumor cells in melanoma patients by analysis of melanoma-associated transcripts, especially tyrosinase. Since the development of the first PCR method for tyrosinase mRNA, several studies have presented varying results. LÄS MER

7. 5. Plant Aquaporins: A study of expression, localization, specificity, and regulation

   Författare :Sara Sjövall Larsen; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Växtbiokemi; Plant biochemistry; phosphorylation; protein kinase; overexpression; NIP; MIP; plant; aquaporin;

   Sammanfattning : Aquaporins, or Major Intrinsic Proteins (MIPs), are integral proteins that facilitate transport of water and other small neutral solutes across biological membranes. They belong to a well conserved and ancient family of proteins, present in all organisms ranging from bacteria to plants and humans. LÄS MER