Sökning: "qPCR"

Visar resultat 6 - 10 av 161 avhandlingar innehållade ordet qPCR.

1. 6. Improved diagnosis and prediction of community-acquired pneumonia

   Författare :Alicia Edin; Anders F. Johansson; Anders Sjöstedt; Annika Allard; Thomas Schön; Umeå universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Community-acquired pneumonia; infection; diagnosis; qPCR; metabolites; metabolomics;

   Sammanfattning : Community-acquired pneumonia (CAP) is a major cause of morbidity and mortality worldwide. Although there is wide variation in the microbial etiology, CAP may manifest with similar symptoms, making institution of proper treatment challenging. LÄS MER

3. 7. Mechanisms of Osseointegration: Experimental Studies on Early Cellular and Molecular Events in vivo

   Författare :Omar Omar; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Osseointegration; titanium; bone; gene expression; bone-implant interface; qPCR; inflammation; cytokine; chemotaxis; chemokine; integrin; bone formation; remodeling; in vivo; rat; immunohistochemistry; ultrastructure; biomechanical torque test;

   Sammanfattning : The early cellular and molecular activities determining the early tissue response and bone formation at bone/implant interface are not fully understood. The general aim of the current thesis was to develop a model for studying the early molecular and cellular activities in different bone types, and in response to different implant surface properties. LÄS MER

4. 8. Effect of Probiotics on Caries-related Variables

   Författare :Sahal Alforaidi; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bacterial interference; ; Caries; Caries prevention; ; Dental biofilm; ; Dental plaque; ; Lactobacilli; Lactobacillus reuteri; ; Streptococcus mutans; ; Plaque acidogenicity; ; Probiotics; qPCR; Saliva; Virulence;

   Sammanfattning : In relation to the caries disease, the aims of this thesis were to investigate the effect of probiotic drops on different caries-related variables in vivo (Papers I & III), on interference capability and genetic response to different metabolites in vitro (II) and systematically to review the best mode and dose of administration based on the examined oral outcomes (IV). The effects on colonisation and cariogenic bacteria were studied in plaque and saliva via plating and qPCR-analyses in teenagers and orthodontic patients after short-term exposure. LÄS MER

5. 9. Composition and dynamics of the bacterial community in aerobic granular sludge reactors

   Författare :Enikö Barbara Szabo; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; qPCR; sequencing batch reactors; microbial community composition; T-RFLP; functional groups; Illumina MiSeq; wastewater; aerobic granular sludge; nitrogen removal; population dynamics;

   Sammanfattning : The aerobic granular sludge (AGS) technology is probably the future standard for wastewater treatment, due to its low footprint and low energy consumption. Although achieving granulation is usually not a challenge anymore, our understanding of the community assembly during start-up, and of the microbial ecology of these reactors in general, is incomplete. LÄS MER

7. 10. Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays

   Författare :Shaman Muradrasoli; Jonas Blomberg; Göran Friman; Maria Brytting; Uppsala universitet; []
   Nyckelord :Virology; Diagnosis of Viral RNA; QPCR; RNA virus; broadly targeted PCR; probe design strategy; Infectious diseases; Medicin; Medicine;

   Sammanfattning : As the area of nucleic acid based technologies develops, so will our understanding of how structural variations in DNA and RNA pathogens are associated with disease. The overall goal of this thesis is the development of broadly targeted measurement techniques for variable viral RNA by Real-Time PCR (here referred to as quantitative reverse transcriptase PCR, QRT-PCR). LÄS MER