Sökning: "proteomik"

Visar resultat 6 - 10 av 78 avhandlingar innehållade ordet proteomik.

  1. 6. Protein based approaches to understand and prevent contagious bovine pleuropneumonia

    Författare :Carl Hamsten; Anja Persson; Joachim Frey; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; CBPP; Mycoplasma mycoides subsp. mycoides small colony type; humoral immune responses; recombinant surface proteins; ELISA; subunit vaccine; suspension bead array; Bioengineering; Bioteknik;

    Sammanfattning : Contagious bovine pleuropneumonia (CBPP) is a severe infectious disease caused by Mycoplasma mycoides subsp. mycoides small colony type (M. mycoides SC) and is a vast problem in Africa. LÄS MER

  3. 7. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

    Författare :My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

    Sammanfattning : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. LÄS MER

  4. 8. Epitope mapping of antibodies towards human protein targets

    Författare :Barbara Hjelm; Mathias Uhlén; Larry Gold; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; antibody; antibody validation; biomarker; epitope mapping; peptide array; proteomics; RBM3; staphylococcal surface display;

    Sammanfattning : This thesis, based on five research papers, presents results from development and evaluation ofmethods for identifying the interaction site of antibodies on their antigens and the functional investigation of these in different assays. As antibodies have proven to be invaluable tools in diagnostics, therapy and basic research, the demand of characterizing these binding molecules has increased. LÄS MER

  5. 9. Development of a covalent site-specific antibody labeling strategy by the use of photoactivable Z domains

    Författare :Anna Konrad; Sophia Hober; Hjalmar Brismar; Patrik Samuelsson; KTH; []
    Nyckelord :antibody labeling; site-specific; covalent; SPPS; Z domain; BPA; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : The joining of two molecular functions or the strategy of adding functions to proteins has been tremendously important for the development of proteins as tools in research and clinic. Depending on the intended application, there are a wide variety of functions that can be added to a proteins. LÄS MER

  7. 10. Generation and characterization of antibodies for proteomics research

    Författare :Karin Larsson; Sophia Hober; John McCafferty; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; antibody; antibody validation; immunohistochemistry; immunofluorescence; tissue microarray; Western blot; epitope mapping; antigen design; Bioengineering; Bioteknik;

    Sammanfattning : Specific antibodies are invaluable tools for proteomics research. The availability of thoroughly validated antibodies will help to improve our understanding of protein expression, localization and function; fundamental processes and features of all living organisms. LÄS MER