Sökning: "proteomics"

Visar resultat 16 - 20 av 430 avhandlingar innehållade ordet proteomics.

  1. 16. Chemical labelling methods for gel free based proteomics

    Författare :Åsa Wåhlander; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biotechnology; Bioteknik; Biokemisk teknik; Biochemical technology; 2D-HPLC; isotopes; labelling; proteomics; mass spectrometry;

    Sammanfattning : In this thesis different aspects of gel free procedures for mass spectrometry based proteome analysis are addressed, with emphasis on labelling techniques. It is based on three original articles where the effects imposed onto peptides upon modification are investigated, and the development of a new type of labelling strategy is described. LÄS MER

  3. 17. Proteomics and metabolomics in biological and medical applications

    Författare :Liudmila Shiryaeva; Wolfgang P. Schröder; Pernilla Wikström; Peter Roepstroff; Umeå universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; 2D-DIGE; biomarkers; cold-acclimation; conifer; freezing tolerance; GC-MS; metabolomics; multiple hypothesis test; multivariate analysis; OPLS-DA; Picea obovata; plasma; prostate cancer; proteomics; ProteoMiner; Siberian spruce;

    Sammanfattning : Biological processes in living organisms consist of a vast number of different molecular networks and interactions, which are complex and often hidden from our understanding. This work is focused on recovery of such details for two quite distant examples: acclimation to extreme freezing tolerance in Siberian spruce (Picea obovata) and detection of proteins associated with prostate cancer. LÄS MER

  4. 18. The accuracy of statistical confidence estimates in shotgun proteomics

    Författare :Viktor Granholm; Lukas Käll; Alexey Nesvizhskii; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteomics; Peptides; Statistics; Mass spectrometry; Tandem mass spectrometry; Biochemistry towards Bioinformatics; biokemi med inriktning mot bioinformatik;

    Sammanfattning : High-throughput techniques are currently some of the most promising methods to study molecular biology, with the potential to improve medicine and enable new biological applications. In proteomics, the large scale study of proteins, the leading method is mass spectrometry. LÄS MER

  5. 19. New Proteomics Methods and Fundamental Aspects of Peptide Fragmentation

    Författare :Mikhail Savitski; Roman Zubarev; Per Andren; David Fenyö; Uppsala universitet; []
    Nyckelord :Bioinformatics; Proteomics; Peptide fragmentation; Bioinformatik;

    Sammanfattning : The combination of collision-activated dissociation, (CAD) and electron capture dissociation, (ECD) yielded a 125% increase in protein identification. The S-score was developed for measuring the information content in MS/MS spectra. This measure made it possible to single out good quality spectra that were not identified by a search engine. LÄS MER

  7. 20. Proteomics informed investigation of human hepatocytes and liver tissue

    Författare :Niklas Handin; Per Artursson; Peter Nygren; Tobias Sjöblom; Mårten Fryknäs; Pieter Annaert; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; human; liver; hepatocytes; proteomics; cellular stress; apoptosis; hepatotoxicity; 3D culture; spheroid; drug metabolism; drug toxicity; drug transport; Farmaceutisk vetenskap; Pharmaceutical Science;

    Sammanfattning : A successful drug needs to display beneficial absorption, distribution, metabolism, excretion and toxicity (ADME-Tox) profile. It is therefore important to investigate these properties during the drug discovery process. LÄS MER