Sökning: "protein translation"

Visar resultat 1 - 5 av 307 avhandlingar innehållade orden protein translation.

1. 1. Evolutionary Mechanisms Shaping Bacterial Translation Termination

   Författare :Shreya Pundir; Suparna Sanyal; Martin Ott; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Ribosome; Protein; Release Factor; Translation; Termination; Stop codon; Evolution; Mutation; Kinetics; Cryo-EM; Molekylär biovetenskap; Molecular Life Sciences;

   Sammanfattning : Translation termination in bacteria involves precise reading of stop codons (UAA, UAG, UGA) and coordinated peptidyl-tRNA hydrolysis by the class-I release factors (RFs) on the ribosome (70S). This thesis investigates the evolutionary and post-translational modification mechanisms of these RFs and the concurrent effect on bacterial translation termination. LÄS MER

3. 2. Proteome wide protein production

   Författare :Hanna Tegel; Sophia Hober; Diethard Mattanovich; KTH; []
   Nyckelord :protein production; Escherichia coli; transcription; promoter; translation; rare codon; high-throughput; screening;

   Sammanfattning : Over a decade after the completion of the human genome, researchers around the world are still wondering what information is hidden in the genome. Although the sequences of all human genes are known, it is still almost impossible to determine much more than the primary protein structure from the coding sequence of a gene. LÄS MER

4. 3. The Protein Traffic on the Ribosome : The Mechanism and Regulation of Protein Synthesis in Prokaryotes

   Författare :Andrey Zavialov; Måns Ehrenber; Venki Ramakrishnan; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; yellow; ribosome; translation; Gagarin; nobel prize; Biokemi; Biochemistry; Biokemi;

   Sammanfattning : The aim of this work was to understand the molecular mechanism of translation and the mechanism of translation termination, in particular. Cleavage of peptidyl-tRNA and peptide release terminates translation of mRNA on the ribosome. In prokaryotes, three release factors (RFs) are involved in this process. LÄS MER

5. 4. Adaptive Evolution of the Bacterial Translation Machinery

   Författare :Arindam De Tarafder; Suparna Sanyal; Michael Y. Pavlov; Ruben L. Jr. Gonzalez; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribosome; Translation; Translation machinery; Elongation; Evolution; RNA world; Cold bacteria; Molekylär biovetenskap; Molecular Life Sciences;

   Sammanfattning : The process of protein synthesis via translation is of paramount importance for the existence of life on Earth. The bacterial translation machinery has embraced more than 3.5 billion years of molecular evolution to adapt and function efficiently under the provided physiological conditions. LÄS MER

7. 5. Versatile Implementations of an Improved Cell-Free System for Protein Biosynthesis : Functional and structural studies of ribosomal protein L11 and class II release factor RF3. Novel biotechnological approach for continuous protein biosynthesis

   Författare :Lamine Bouakaz; Måns Ehrenberg; Richard Buckingham; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Protein synthesis; translation termination; ribosome; release factor; cell-free system; missense error; gel filtration; affinity chromatography; Molekylärbiologi; Molecular biology; Molekylärbiologi;

   Sammanfattning : Advances in genetics, proteomics and chromatography techniques have enabled the successfully generation of a cell-free bacterial translation system composed of highly pure and active components. This system provided an ideal platform for better elucidating the mechanism of each individual step of the prokaryotic protein biosynthesis and the function of the translation factors involved in the process. LÄS MER