Sökning: "protein synthesis"

Visar resultat 21 - 25 av 939 avhandlingar innehållade orden protein synthesis.

1. 21. In silico protein design for the enhancement of protein stability and function

   Författare :David A. Hueting; Per-Olof Syrén; Hjalmar Brismar; Zbynek Prokop; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Protein Engineering; Ancestral sequence reconstruction; terpene cyclases; SARS-CoV-2; Spike protein; PET; PETase; rational engineering; Proteinteknik; Förfäders sekvensrekonstruktion; Terpencyklas; SARS-CoV-2; Spike protein; PET; PETas; rationell proteinteknik; Kemi; Chemistry;

   Sammanfattning : Enzymes are natures catalysts that increase the rate of a chemical reaction. The increased rate of a reaction is required to be able to sustain life. Despite the huge impact of enzymes, they are not perfect catalysts. LÄS MER

3. 22. Rate and Accuracy of Bacterial Protein Synthesis with Natural and Unnatural Amino Acids

   Författare :Ka-Weng Ieong; Måns Ehrenberg; Simpson Joseph; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribosome; protein synthesis; translation; efficiency-accuracy trade-off; kinetics; unnatural amino acids; Molecular Biology; Molekylärbiologi;

   Sammanfattning : This thesis addresses different questions regarding the rate, efficiency, and accuracy of peptide bond formation with natural as well as unnatural amino acids: Which step is rate-limiting during peptide bond formation? How does the accuracy vary with different transfer RNAs (tRNAs) and codons and how is it relevant to the living cells? Does proofreading selection of codon reading occur in a single- or multi-step manner as theoretically suggested? How does the E. coli translation system discriminate unnatural amino acids? Based on that, how to improve the incorporation efficiencies of unnatural amino acids?Based on the study on pH dependence of peptide bond formation, we show that the rate of the chemistry of peptidyl transfer to aminoacyl-tRNA (AA-tRNA) Gly-tRNAGly or Pro-tRNAPro limits the rate of peptide bond formation at physiological pH 7. LÄS MER

4. 23. Insights Into Protein Synthesis - Structural Aspects of Elongation Factor G and Fusidic Acid Inhibition

   Författare :Sebastian Hansson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molekylär biofysik; Structure chemistry; Strukturkemi; Molecular biophysics; Biologi; Biology; Biomedicinska vetenskaper; Biomedical sciences; conformational change; fusidic acid; protein synthesis; elongation factor G; crystal structures;

   Sammanfattning : Elongation factor G (EF-G) catalyses the translocation step in protein synthesis on the ribosome. During this event the antibiotic Fusidic acid (FA) binds to and irreversably inhibits the release of the EF-G:GDP complex from the ribosome. LÄS MER

5. 24. Design, Synthesis and Characterization of Small Molecule Inhibitors and Small Molecule : Peptide Conjugates as Protein Actors

   Författare :Jonas Nilsson; Lars Baltzer; Ingemar Kvarnström; Thomas Olsson; Linköpings universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Synthetic receptor; Peptide inhibitor conjugate; Peptide protein surface interactions; General Lead Discovery Library; Combinatorial Chemistry; Solid Phase Chemistry; Targeted Library; Thrombin inhibitor; Fluorescence; Surface Plasmon Resonance; Organic chemistry; Organisk kemi;

   Sammanfattning : This thesis describes different aspects of protein interactions. Initially the function of peptides and their conjugates with small molecule inhibitors on the surface of Human Carbonic Anhydrase isoenzyme II (HCAII) is evaluated. LÄS MER

7. 25. Organization of mitochondrial gene expression in yeast : Specific features of organellar protein synthesis

   Författare :Kirsten Kehrein; Martin Ott; Thomas A Fox; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mitochondria; translation; bc1 complex; ribosome; gene expression; Biochemistry; biokemi;

   Sammanfattning : Mitochondria contain their own genetic system, encoding key subunits of the oxidative phosphorylation system. These subunits are expressed by an organelle-specific gene expression machinery. LÄS MER