Sökning: "protein stability"

Visar resultat 6 - 10 av 588 avhandlingar innehållade orden protein stability.

1. 6. Sweet stability

   Författare :Kajsa Ahlgren; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; myoglobin; lysozyme; amyloid fibrils; DSC; trehalose; protein denaturation; neutron scattering; glass transition; sucrose;

   Sammanfattning : The disaccharide trehalose is known for its ability to stabilise proteins by preventing aggregation and elevating their denaturation temperature, consequently avoiding unfolding. The folding and function of proteins are vital for many biological processes. LÄS MER

3. 7. The birth and growth of the protein folding nucleus : Studies of protein folding focused on critical contacts, topology and ionic interactions

   Författare :Linda Hedberg; Mikael Oliveberg; Christopher Johnson; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein folding; protein stability; two-state folding; chevron plot; transition-state movements; Hammond behaviour; topology; electrostatic interactions; pH dependence; mass-action; protein engineering; pKa; Biochemistry; Biokemi; biokemi; Biochemistry;

   Sammanfattning : Proteins are among the most complex molecules in the cell and they play a major role in life itself. The complexity is not restricted to just structure and function, but also embraces the protein folding reaction. LÄS MER

4. 8. Folding of the Ribosomal protein S6 : The role of sequence connectivity, overlapping foldons, and parallel pathways

   Författare :Ellinor Haglund; Mikael Oliveberg; Neil Ferguson; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein folding; protein stability; two-state folding; S6; chevron plot; transition state; parallell pathways; foldon; two-channel landscape; protein engineering; H D-exchange; Biochemistry; Biokemi; Biochemistry; biokemi;

   Sammanfattning : To investigate how protein folding is affected by sequence connectivity five topological variants of the ribosomal protein S6 were constructed through circular permutation.  In these constructs, the chain connectivity (i.e. the order of secondary-structure elements) is changed without changing the native-state topology. LÄS MER

5. 9. Structural and thermodynamical basis for molecular recognition between engineered binding proteins

   Författare :Jakob Dogan; Per-Åke Nygren; John E. Ladbury; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein structure; induced fit; binding thermodynamics; NMR spectroscopy; protein engineering; protein-protein interactions; protein stability; calorimetry; Structural biochemistry; Strukturbiokemi;

   Sammanfattning : The structural determination of interacting proteins, both as individual proteins and in their complex, complemented by thermodynamical studies are vital in order to gain in-depth insights of the phenomena leading to the highly selective protein-protein interactions characteristic of numerous life processes. This thesis describes an investigation of the structural and thermodynamical basis for molecular recognition in two different protein-protein complexes, formed between so-called affibody proteins and their respective targets. LÄS MER

7. 10. Protein stability and mobility in live cells : Revelation of the intracellular diffusive interaction organization mechanisms

   Författare :Xin Mu; Mikael Oliveberg; Johan Elf; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; diffusive interactions; macromolecular crowding; protein thermodynamic stability; protein mobility; in-cell NMR; Hofmeister series; Biochemistry; biokemi;

   Sammanfattning : Biochemical processes inside living cells take place in a confined and highly crowded environment. As such, macromolecular crowding, one of the most important physicochemical properties of cytoplasm, is an essential element of cell physiology. LÄS MER