Sökning: "protein reconstitution"

Visar resultat 1 - 5 av 45 avhandlingar innehållade orden protein reconstitution.

  1. 1. Perplexing Protein Puzzles

    Författare :Stina Lindman; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; calbindin D9k; split-GFP; protein G B1; pKa-values; protein reconstitution; Electrostatic interactions; protein stability; protein denaturation; heteronuclear NMR; pH titrations;

    Sammanfattning : Protein structure and stability are inherent in the amino acid sequence and governed by non-covalent interactions. The cooperation between forces is, however, perplexing and not well understood. In order to elucidate and predict protein folding and stability, detailed studies of non-covalent interactions are required. LÄS MER

  2. 2. Unravelling protein stability and aggregation

    Författare :Tanja Weiffert; NanoLund; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; protein stability; protein aggregation; amyloid beta peptide; protein stabilization; split GFP; monellin; secretagogin; ; aggregation kinetics; toxicity; aggregation mechanism; coaggregation;

    Sammanfattning : Proteins are complex macromolecules that are fundamental to all living species. The stability of proteins are governed by non-covalent forces within the protein and between the protein and its surrounding environment. LÄS MER

  3. 3. To Fold or To Fibrillate? Serendipity in Stability Studies

    Författare :Olga Szczepankiewicz; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Protein stability; protein reconstitution; protein aggregation; Monellin; PGB1; Split-GFP; Amyloid beta;

    Sammanfattning : Proteins are complex structures and years of research have been spent on attempts to understand their complexity. The non-covalent interactions involved in protein folding are: hydrophobic effect, electrostatic interactions, van der Waals interactions and hydrogen bonding. LÄS MER

  4. 4. Insight into the mitochondrial apoptotic pathway : The interplay of the pro-apoptotic Bax protein with oxidized phospholipids and its counterplayer, the pro-survival Bcl-2 protein

    Författare :Marcus Wallgren; Gerhard Gröbner; Marco Colombini; Umeå universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; apoptosis; Bax; Bcl-2; CD; cell-free protein synthesis; DSC; membrane; mitochondria; oxidized phospholipids; reconstitution; solid state NMR; biologisk kemi; biological chemistry;

    Sammanfattning : Apoptosis plays a crucial role in multicellular organisms by preserving tissue homeostasis and removing harmful cells. The anti-apoptotic B-cell CLL/lymphoma 2 (Bcl-2) and the pro-apoptotic Bcl-2-associated X protein (Bax) act as major regulators of the mitochondrial apoptotic pathway. LÄS MER

  5. 5. Membrane-mimetic systems : Novel methods and results from studies of respiratory enzymes

    Författare :Gustav Nordlund; Peter Brzezinski; Joachim Heberle; Stockholms universitet; []
    Nyckelord :NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Lipids; membrane proteins; method development; respiration; reconstitution; supported membranes; SNAREs; liposome fusion; lipid-protein interactions; biokemi; Biochemistry;

    Sammanfattning : The processes localized to biological membranes are of great interest, both from a scientific and pharmaceutical point of view. Understanding aspects such as the detailed mechanism and regulation of these processes requires investigation of the structure and function of the membrane-bound proteins in which they take place. LÄS MER