Sökning: "protein quantification"

Visar resultat 21 - 25 av 210 avhandlingar innehållade orden protein quantification.

1. 21. Plant Membrane Proteomics - using isolated membranes and proteins to compare and quantify subproteomes

   Författare :Katja Bernfur; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; quantification; proteomics; metabolic labeling; plasma membrane;

   Sammanfattning : Membrane proteins are particularly important to characterize, since they are involved in cellular processes of utmost importance in control and regulation of cells, such as transport across membranes, signal transduction and photosynthesis. The word "proteome" means all proteins expressed by a e.g. LÄS MER

3. 22. Characterization of biological nanoparticles using evanescent field sensing

   Författare :Mattias Sjöberg; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; label-free; protein corona; mass quantification.; liposomes; single-particle; SPR; Waveguide scattering microscopy;

   Sammanfattning : In light of the increasingly realized importance of nanoparticle-based biological processes, in biology as well as in technological applications, there is a need of analysis methods capable of accurately quantifying different characteristics and processes involving these elusive entities. The aim of this thesis is the development and utilization of surface-based bioanalytical sensing methods for quantitative characterization of biological nanoparticles, most of which being based on surface-confined evanescent-field illumination. LÄS MER

4. 23. Analysis of Complex Biological Samples using Liquid Chromatography-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

   Författare :Margareta Ramström; Jonas Bergquist; Per Håkansson; Karin Markides; Carol Robinson; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Analytical chemistry; mass spectrometry; liquid chromatography; protein; proteomics; peptide; Fourier transform ion cyclotron resonance mass spectrometry; cerebrospinal fluid; amyotrophic lateral sclerosis; electrospray ionization; electron capture dissociation; Analytisk kemi; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Studies of protein and peptide expression are vital in order to understand complex biological systems. As demonstrated in this thesis, on-line packed capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR MS) is a useful analytical tool for such studies. LÄS MER

5. 24. Analysis of proteins related toautoimmune and inflammatorydiseases with focus on newenrichment methodologies and MALDI-TOF MS

   Författare :Yuye Zhou; Åsa Emmer; Margret Thorsteinsdottir; KTH; []
   Nyckelord :Protein; Glycopeptide; IgG; Osteopontin; Enrichment; Digestion; Separation; Microchip; MALDI-TOF MS; Kemi; Chemistry;

   Sammanfattning : Proteins have been widely studied in biological sample analysis due to the association with diseases. In the present project, method and technique development was carried out on the analysis of two proteins, immunoglobulin G (IgG) and osteopontin (OPN). LÄS MER

7. 25. Method Development in Quantitative and Structural Proteomics using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

   Författare :Charlotte Hagman; Per Håkansson; Jonas Bergquist; Jos Buijs; Michael Przybylski; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Cerebrospinal Fluid; Electrospray Ionization; Fourier Transform Ion Cyclotron Resonance Mass Spectrometry; Global Markers; High Performance Liquid Chromatography; Gas-Phase Exchange; Hydrogen Deuterium Exchange; Plasma; Protoemics; Quantification; Structural Elucidation; Bioteknik; Bioengineering; Bioteknik;

   Sammanfattning : In this thesis, methods for studying different aspects of proteomics were developed with Fourier Transform Ion Cyclotron Resonance, (FTICR), mass spectrometry. The FTICR technique provides ultra-high mass resolving power, mass accuracy at sub ppm level and sensitivity in the attomole region. LÄS MER