Sökning: "protein labelling"

Visar resultat 1 - 5 av 70 avhandlingar innehållade orden protein labelling.

  1. 1. Engineering of small IgG binding domains for antibody labelling and purification

    Författare :Sara Kanje; Sophia Hober; Cecília Roque; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Antibody; labelling; purification; Protein G; Protein A; protein engineering; protein design; combinatorial selection; Bioteknologi; Biotechnology;

    Sammanfattning : In protein engineering, rational design and selection from combinatorial libraries are methods used to develop proteins with new or improved features. A very important protein for the biological sciences is the antibody that is used as a detecting agent in numerous laboratory assays. LÄS MER

  3. 2. Protein phosphorylation in plant mitochondria

    Författare :André Struglics; Lund Ortopedi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; FoF1-ATPase; autophosphorylation; protein kinase activity; protein phosphorylation; Plant mitochondria; inner mitochondrial membrane; NDPK; Physiological biophysics; Växtfysiologi;

    Sammanfattning : Protein phosphorylation in the subcompartments of plant mitochondria was investigated by labelling with [gamma-32P]ATP and by SDS-PAGE/autoradiography. About 20 proteins in inside-out inner mitochondrial membranes from potato tubers were phosphorylated by endogenous protein kinases when incubated with [gamma-32P]ATP. LÄS MER

  4. 3. Chemical labelling methods for gel free based proteomics

    Författare :Åsa Wåhlander; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biotechnology; Bioteknik; Biokemisk teknik; Biochemical technology; 2D-HPLC; isotopes; labelling; proteomics; mass spectrometry;

    Sammanfattning : In this thesis different aspects of gel free procedures for mass spectrometry based proteome analysis are addressed, with emphasis on labelling techniques. It is based on three original articles where the effects imposed onto peptides upon modification are investigated, and the development of a new type of labelling strategy is described. LÄS MER

  5. 4. Tumour Targeting using Radiolabelled Affibody Molecules : Influence of Labelling Chemistry

    Författare :Mohamed Altai; Vladimir Tolmachev; Jacques Barbet; Uppsala universitet; []
    Nyckelord :HER2; Affibody molecule; Radionuclide molecular maging; Targeted radionuclide therapy; Labeling chemistry;

    Sammanfattning : Affibody molecules are promising candidates for targeted radionuclide-based imaging and therapy applications. Optimisation of targeting properties would permit the in vivo visualization of cancer-specific surface receptors with high contrast. In therapy, this may increase the ratio of radioactivity uptake between tumour and normal tissues. LÄS MER

  7. 5. Membrane protein proteomics - Novel method for membrane protein identification and quantification

    Författare :Maria Bentz; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biokemisk teknik; mebran; steroider; Lipider; membranes; steroids; Lipids; enzymologi; Proteiner; Membrane proteins; Proteomics; Proteinase K; N-terminal labelling; Relative Quantitation; Database search; Cancer; Cytology; oncology; cancerology; Cytologi; onkologi; cancer; enzymology; Proteins; Biochemical technology;

    Sammanfattning : Membrane proteins are fairly refractory to digestion especially by trypsin. Less specific proteases like elastase and pepsin are much more effective. However database searching using non-tryptic peptides is much less effective due to the lack of charge localisation at the N- and C-termini and the absence of sequence specificity. LÄS MER