Sökning: "protein identification techniques"

Visar resultat 1 - 5 av 76 avhandlingar innehållade orden protein identification techniques.

1. 1. Identification and Characterization of Peptides and Proteins using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

   Författare :Magnus Palmblad; Ole Nørregaard Jensen; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Materials science; Peptide; protein; peptide mass fingerprinting; identification; sequencing; protein structure; non-covalent interaction; modification; electrospray ionization; liquid chromatography; capillary electrophoresis; electron capture dissociation; Fourier transform ion cyclotron resonance mass spectrometry; cerebrospinal fluid; amyloid β-peptide; Alzheimer’s disease.; Materialvetenskap; Materials science; Teknisk materialvetenskap; Molecular Biotechnology; Molekylär bioteknik;

   Sammanfattning : Mass spectrometry has in recent years been established as the standard method for protein identification and characterization in proteomics with excellent intrinsic sensitivity and specificity. Fourier transform ion cyclotron resonance is the mass spectrometric technique that provides the highest resolving power and mass accuracy, increasing the amount of information that can be obtained from complex samples. LÄS MER

3. 2. On bacterial formats in protein library technology

   Författare :Per-Åke Löfdahl; Per-Åke Nygren; Mikael Widersten; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Affibody molecule; β-lactamase; combinatorial library; green fluorescent protein GFP ; protein engineering; protein fragment complementation assay PCA ; selection; TEVprotease; TNF-α; Bioengineering; Bioteknik; Enzyme engineering; Enzymteknik;

   Sammanfattning : Millions of years of evolution have resulted in an immense number of different proteins, which participate in virtually every process within cells and thus are of utmost importance for allknown forms of life. In addition, there are several examples of natural proteins which have found use in applications outside their natural environment, such as the use of enzymes infood industry and washing powders or the use of antibodies in diagnostic, bioseparation or therapeutic applications. LÄS MER

4. 3. New methods for protein structure threading and domain identification

   Författare :Mats Lindstam; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; biomatematik; Molecular biophysics; Molekylär biofysik; medicinsk informatik; Bioinformatik; biomathematics biometrics; medical informatics; structure prediction; Bioinformatics; domain identification; Structural bioinformatics; threading;

   Sammanfattning : The availability of three-dimensional structures of proteins has provided enormous insight into the detailed molecular machinery of the living cell. Since the late 1950s and early 1960s, experimental methods for deriving protein structures have contributed more than 30,000 entries to the Protein Data Bank, representing the collected knowledge of protein and other macromolecular structures. LÄS MER

5. 4. Bioimaging for analysis of protein expression in cells and tissues using affinity reagents

   Författare :Emma Lundberg; Torbjörn Gräslund; Mathias Uhlén; Rainer Pepperkok; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; Affibody; antibody; biomarker; cell line; cell microarray; colorectal cancer; confocal microscopy; HER2; immunohistochemistry; immunofluorescence; light microscopy; protein expression; protein localization; SATB2; tissue microarray; Bioengineering; Bioteknik; Cell and molecular biology; Cell- och molekylärbiologi;

   Sammanfattning : The detection and analysis of biomolecules, such as proteins, are of great interest since these molecules are fundamental for life and our health. Due to the complexity of biological processes, there is a great advantage of studying proteins in their natural context, for example by using bioimaging. LÄS MER

7. 5. Protein Profiling and Type 2 Diabetes

   Författare :Tea Sundsten; Peter Bergsten; Leif Jansson; Maria Sörhede Winzell; Uppsala universitet; []
   Nyckelord :Cell biology; Type 2 diabetes; Protein profiling; SELDI-TOF MS; Proteomics; Cellbiologi;

   Sammanfattning : Type 2 diabetes mellitus (T2DM) is a heterogeneous disease affecting millions of people worldwide. Both genetic and environmental factors contribute to the pathogenesis. The disease is characterized by alterations in many genes and their products. LÄS MER