Sökning: "protein folding chalmers"

Visar resultat 1 - 5 av 7 avhandlingar innehållade orden protein folding chalmers.

1. 1. Systems biology of protein synthesis and secretion in yeast

   Författare :Qi Qi; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; recombinant protein production; protein synthesis; multi-omics analysis; protein secretion; genome-scale modeling;

   Sammanfattning : Protein synthesis and secretion is a vital process to maintain cell function. As it demands numerous building blocks, cofactors and chaperones generated from metabolism and translation, the process is intertwined with metabolic and regulatory networks. LÄS MER

3. 2. Folding and Structure of Azurin - The Influence of a Metal

   Författare :Johan Leckner; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; azurin; metal cofactor; disulfide bond; protein folding; solution structure; protein stability; paramagnetic NMR;

   Sammanfattning : The structural role of the metal in the protein azurin from Pseudomonas aeruginosa has been a long standing question. Azurin belongs to the cupredoxin family and is a 128 residue beta-barrel protein of greek-key topology. LÄS MER

4. 3. Protein modelling by the zipping and assembly method with limited NMR-derived constraints

   Författare :Maryana Wånggren; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Molecular dynamics simulations; protein folding; constraints;

   Sammanfattning : Molecular dynamics simulations, often combined with simulated annealing, are commonly used when calculating structural models of proteins, e.g. based on NMR experiments. However, one is often faced with limited and, sometimes, insufficient information for determining a well-resolved 3D structure. LÄS MER

5. 4. Metabolic Engineering of Recombinant Protein Production by Saccharomyces cerevisiae

   Författare :Zihe Liu; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; recombinant protein production; genome sequencing; DNA microarray; insulin precursor; growth rate; UV mutation; yeast; anaerobic electron acceptor; secretory pathway; unfolded protein response; metabolic engineering; systems biology; α-amylase;

   Sammanfattning : The yeast Saccharomyces cerevisiae is a widely used cell factory for the production of fuels, chemicals, and it also provides a platform for the production of many heterologous proteins of medical or industrial interest. In this thesis, random and rational approaches, such as vector design, host engineering, fermentation analysis, UV Mutation, coupled with high-throughput systems biology techniques (including whole genomic sequencing, microarray analysis and flux analysis) and integrated analysis (Reporter feature technique), were employed to engineer cellular properties more effectively and purposefully to construct cell factories for protein production. LÄS MER

7. 5. Protein unfolding by interfaces and interactions between polycyclic aromatic hydrocarbons

   Författare :Svetla Chakarova Käck; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; first-principles calculations; PAH; denaturation; protein-interface interactions; van der Waals interactions; lattice models; polycyclic aromatic hydrocarbons; DFT; density functional theory;

   Sammanfattning : In order to theoretically study large and complex systems, a variety of approaches that can be categorized loosely as top-down or bottom-up are followed. While our vision is to bridge the gap between these approaches and use them together in multiscale materials modeling, the present thesis is limited to give examples of each type of approach in the study of soft-matter systems. LÄS MER