Sökning: "protein corona"

Visar resultat 1 - 5 av 16 avhandlingar innehållade orden protein corona.

  1. 1. Bioanalysis using capillary electrophoresis and mass spectrometry : Applied on proteins, protein nanofibrils and polyvinyl alcohol microbubbles

    Författare :Leila Josefsson; Åsa Emmer; Ulrika Nilsson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; : amyloid; capillary electrophoresis; contrast agents; detection; human blood plasma; ionic liquids; mass spectrometry; microbubbles; polyvinyl alcohol microbubbles; protein nanofibrils; proteins; Kemi; Chemistry;

    Sammanfattning : The sequencing of the genome of various species, including the human species, have led to increased understanding about how a protein structure is generated, and how specific structures are related to the proteins’ functionality. In paper I and II of this thesis, the folding of proteins in vitro to form hierarchical nanostructures, which in vivo often have a pathological effect, have been studied. LÄS MER

  3. 2. Nanostructure and biomolecule interactions : Characterizing the complex

    Författare :Stefan Gunnarsson; NanoLund: Centre for Nanoscience; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Nanostructures; Proteins; Lipids; Protein corona; Aggregation; Sedimentation; Diffusion; Electron microscopy; Enzyme activity; Nanowires; Nanoparticles;

    Sammanfattning : This thesis presents the results of studies done on various biomolecules and their interactions with nanomaterials. The biomolecule sources are everything from purified, single proteins to the complicated mixture of blood serum and cell culture media. LÄS MER

  4. 3. Multiparametric Optical Characterization of Biological Nanoparticles using Evanescent Field Sensing

    Författare :Mattias Sjöberg; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein corona; single-particle; liposomes; SPR; lipid nanoparticles; Waveguide scattering microscopy; label-free; mass quantification.;

    Sammanfattning : In light of the increasingly realized dependence of many biological functions on nanoscopic supramolecular assemblies, also including novel biotechnological applications, there is a need for advanced analysis methods capable of accurately quantifying different characteristics of these elusive entities. The prime aim of this thesis is the development and utilization of surface-based bioanalytical sensing methods for quantitative characterization of biological nanoparticles. LÄS MER

  5. 4. Characterization of biological nanoparticles using evanescent field sensing

    Författare :Mattias Sjöberg; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; label-free; protein corona; mass quantification.; liposomes; single-particle; SPR; Waveguide scattering microscopy;

    Sammanfattning : In light of the increasingly realized importance of nanoparticle-based biological processes, in biology as well as in technological applications, there is a need of analysis methods capable of accurately quantifying different characteristics and processes involving these elusive entities. The aim of this thesis is the development and utilization of surface-based bioanalytical sensing methods for quantitative characterization of biological nanoparticles, most of which being based on surface-confined evanescent-field illumination. LÄS MER

  7. 5. Analytical Quality by Design Method Development for Vaccine Characterization

    Författare :Lars Geurink; Catharina Sänger van de Griend; Mikael Hedeland; Martijn Schenning; Iulia Oita; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; viral vaccines; adenovirus; influenza; polio; COVID; corona; capillary electrophoresis; analytical quality by design; analytical method development; CGE; icIEF; CZE; crude cell suspension analysis; in-process sample analysis; quality control testing; biopharmaceutical characterization; Analytisk farmaceutisk kemi; Analytical Pharmaceutical Chemistry;

    Sammanfattning : Vaccines that are safe, efficacious, and can be rapidly developed are needed to prevent and to react to emerging global infectious disease threats such as influenza, Polio, and Coronavirus diseases. Fast and reliable analytical methods are required without delay to support vaccine process and product development, characterization, and quality control testing. LÄS MER