Sökning: "protein chromatography"

Visar resultat 1 - 5 av 303 avhandlingar innehållade orden protein chromatography.

  1. 1. Characterizing Chromatography Media : NMR-based Approaches

    Författare :Fredrik Elwinger; István Furó; Stéphane Viel; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; chromatography; nuclear magnetic resonance; NMR; self-diffusion; magic-angle spinning; quantitative; distribution coefficient; pore size; Kemi; Chemistry;

    Sammanfattning : Liquid chromatography is an essential technique in manufacturing biopharmaceuticals where it is used on all scales from analytical applications in R&D to full-scale production. In chromatography the target molecule, typically a protein, is separated and purified from other components and contaminants. LÄS MER

  3. 2. Protein engineering to explore and improve affinity ligands

    Författare :Martin Linhult; KTH; []
    Nyckelord :binding; affinity; human serum albumin HSA ; albumin-binding domain ABD ; affinity chromatography; deamidation; protein A; stabilization; Z-domain; capacity; protein G; cleaning-in-place CIP ; protein engineering; C2 receptor;

    Sammanfattning : In order to produce predictable and robust systems forprotein purification and detection, well characterized, small,folded domains descending from bacterial receptors have beenused. These bacterial receptors, staphylococcal protein A (SPA)and streptococcal protein G (SPG), possess high affinity to IgGand / or HSA. LÄS MER

  4. 3. Fast isolation of proteins from bioreactors using affinity chromatography techniques

    Författare :Eva Pålsson; Avdelningen för klinisk genetik; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; chromatography; protein purification; analyses; dispersion; chromatography matrices; Biochemistry; Biokemi; Metabolism; expanded bed; metabolism; Biotechnology; Bioteknik;

    Sammanfattning : The purification of proteins from raw materials like fermentation broth and other biological sources usually involves several purification steps and is rather time consuming and expensive. This is a major concern in the production of many important protein products e.g. pharmaceuticals such as insulin and blood coagulation factors. LÄS MER

  5. 4. Protein Recovery Using Simulated Moving Bed Technology and Macroporous Chromatographic Support Material

    Författare :Jonatan Andersson; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioteknik; Biotechnology; Chromatography; Simulated Moving Bed; Protein;

    Sammanfattning : Due to its gentle and high resolving power, chromatography is the work horse within protein separation. Still it is an expensive method due to low productivity, diluted product streams, large waste streams and expensive support material. This limits its potential use to areas of high price products. LÄS MER

  7. 5. Adsorption Studies with Liquid Chromatography : Experimental Preparations for Thorough Determination of Adsorption Data

    Författare :Lena Edström; Jonas Bergquist; Lars Hagel; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Liquid chromatography; HPLC; UHPLC; Reversed phase; Preparative chromatography; Adsorption isotherm; Injection profile; Sample pH; pH stable conditions; Peak deformation; Band distortion; Overloaded band; Chiral preparative chromatography; Analytical Chemistry; Analytisk kemi;

    Sammanfattning : Analytical chemistry is a field with a vast variety of applications. A robust companion in the field is liquid chromatography, the method used in this thesis, which is an established workhorse and a versatile tool in many different disciplines. LÄS MER