Sökning: "protein chip"

Visar resultat 11 - 15 av 97 avhandlingar innehållade orden protein chip.

1. 11. Recombinant Enzymes in Pyrosequencing Technology

   Författare :Nader Nourizad; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; apyrase; pyrosequencing; pichia pastoris; protein expression; fusion protein; luciferase; DNA template; clone checking; DNA polymerase;

   Sammanfattning : Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released. LÄS MER

3. 12. Creation of a Nanometer-Scale Toolbox for Molecular Motor Transport-Circuits

   Författare :Richard Bunk; Fasta tillståndets fysik; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Coating and surface treatment; materialteknik; Materiallära; Material technology; Produktionsteknik; Production technology; Semiconductory physics; Halvledarfysik; Proteins; enzymology; Proteiner; enzymologi; reumatologi; Physics; Fysik; muskelsystem; Skelett; rheumatology locomotion; muscle system; Skeleton; Biofysik; Biophysics; Biomedicinska vetenskaper; Biomedical sciences; fluorescence microscopy; EBL; T-profile channel; nano-imprinting; cargo; quantum dot; silane; myosin; actin; motor protein; factory-on-a-chip; toolbox; lab-on-a-chip; molecular motor; nanotechnology; Biokemisk teknik; Biotechnology; Bioteknik; Skikt och ytbehandling; Biochemical technology;

   Sammanfattning : This thesis presents studies of molecular motors in interaction with nm-scale structures, as well as the development of a set of tools that can be used for the construction of custom-designed nano-transportation systems. In our studies, we have used the latest nanoscale technology and combined this with advanced results from chemistry and biomedical sciences. LÄS MER

4. 13. From Single Gene to Whole Genome Studies of Human Transcription Regulation

   Författare :Alvaro Rada-Iglesias; Claes Wadelius; Henk Stunnenberg; Uppsala universitet; []
   Nyckelord :Genetics; transcription; ChIP-chip; genome-wide; transcription factors; in vivo; Genetik;

   Sammanfattning : Transcriptional regulation largely determines which proteins and the protein levels that are found in a cell, and this is crucial in development, differentiation and responses to environmental stimuli. The major effectors of transcriptional regulation are a group of proteins known as transcription factors, which importance is supported by their frequent involvement in mendelian and complex diseases. LÄS MER

5. 14. Mechanisms of Transgene Silencing in Neural Cells -Implications for Ex Vivo Gene Therapy to the Brain

   Författare :Nina Rosenqvist; Neurobiologi; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; neurophysiology; Neurologi; Neurology; neuropsychology; ChIP; rat; transgene; insulator; differentiation; GFP; lentivirus; DNA methylation; histone deacetylation; chromatin; expression; silencing; stem cell; progenitor; gene therapy; neurofysiologi; neuropsykologi; CNS; transplantation;

   Sammanfattning : The use of genetically modifi ed neural stem and progenitor cells could possibly be a future strategy for repairing a brain suffering from neurodegeneration. However, so far it has proven diffi cult to maintain sufficient levels of a transferred gene in suitable cell types, after transplantation. LÄS MER

7. 15. Membrane-Assisted Isoform ImmunoAssay : Separation and determination of protein isoforms

   Författare :Maria Lönnberg; Carl G M Magnusson; Uppsala universitet; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Bioteknik; Bioengineering; Bioteknik; Surface Biotechnology; ytbioteknik;

   Sammanfattning : Proteins exist in a variety of isoforms with minor differences, mostly due to their glycosylation patterns, which can modulate their biological functions. It seems to be of clinical relevance to measure the isoform-distribution.Thesis describes a novel technology named Membrane-Assisted Isoform ImmunoAssay (MAIIA). LÄS MER