Sökning: "promoter"

Visar resultat 21 - 25 av 811 avhandlingar innehållade ordet promoter.

  1. 21. Physiology of Saccharomyces cerevisiae Producing Recombinant Insulin in Continuous Culture

    Författare :Ali Kazemi Seresht; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; unfolded protein response; Keywords: heterologous protein production; prolonged chemostat; transcriptome; insulin analogue precursor; metabolic adaptation; URA3d; plasmid copy number; promoter truncation; high cell density cultivation; TPI1;

    Sammanfattning : The yeast Saccharomyces cerevisiae has widely been used as a host for the production of heterologous proteins. High-level production of heterologous proteins is likely to impose a metabolic burden on the host cell and can thus affect various aspects of cellular physiology. LÄS MER

  3. 22. Hepatitis B virus infection and genomic changes from a long-term perspective

    Författare :Jonas Bläckberg; Sektion III; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Infektioner; Infections; cor promoter; precore; occult infection; HBV; mutation;

    Sammanfattning : Hepatitis B virus is a non-cytopathic virus with a small, circular, partially double-stranded DNA of 3.2 kb. It causes acute and chronic inflammatory liver disease and hepatocellular carcinoma (HCC). Retesting of stored sera from an outbreak of acute hepatitis in 1969-72 allowed identification of 126 cases of acute hepatitis B. LÄS MER

  4. 23. Human Embryonic Stem Cells - Constitutive Gene Expression and Differentiation Towards Definitive Endoderm and Posterior Foregut Endoderm

    Författare :Karin Norrman; Stamcellscentrum (SCC); []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; human embryonic stem cells; diabetes; pancreas; definitive endoderm; foregut endoderm; pancreatic endoderm; constitutive promoter; ACTB; EF1alpha; CMV; PGK; UbC;

    Sammanfattning : Abstract More than 300 million people are suffering from diabetes worldwide and the numbers of diabetic patients are increasing. Today´s treatments mainly rely on daily insulin injections. This help people to control blood glucose levels but still many patients suffer from the many complications associated with diabetes. LÄS MER

  5. 24. Mechanisms of microbial-host interaction during asymptomatic bacteriuria

    Författare :Jenny Grönberg Hernandez; immunologi och glykobiologi - MIG Avdelningen för mikrobiologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Asymptomatic bacteriuria; Urinary tract infection; Escherichia coli 83972; IRF3; TLR4; Evolution; host response; promoter polymorphisms; transcription; P fimbriae; Type 1 fimbriae;

    Sammanfattning : Urinary tract infections (UTIs) present an interesting and relevant model for studying microbial adaptation. After establishing significant numbers, the bacteria either cause severe disease, or an asymptomatic carrier state resembling the normal flora at other mucosal sites. LÄS MER

  7. 25. Next generation tools for microbial cell factories

    Författare :Alister James Cumming; Daniel Daley; Neil Dixon; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Microbial cell factories; Recombinant protein production; Bacteria; Plasmid; Genetic sensor; Inducible promoter; Genetic cassette; biokemi; Biochemistry;

    Sammanfattning : The bacterium E. coli is a popular choice for expressing recombinant proteins. It is easy to culture and many molecular tools for are available for directing protein production. When producing recombinant proteins, high yields and good quality soluble products are desirable. LÄS MER