Sökning: "pre-steady state kinetics"

Hittade 5 avhandlingar innehållade orden pre-steady state kinetics.

  1. 1. Structural and biochemical basis for the high fidelity and processivity of DNA polymerase ε

    Författare :Rais Ahmad Ganai; Erik Johansson; Per Elias; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; DNA polymerase ε; Crystal structure; P domain; Pre-steady state kinetics; β-hairpin loop; Primer switching; Strand displacement; biokemi; Biochemistry;

    Sammanfattning : DNA polymerase epsilon (Pol ε) is a multi-subunit B-family DNA polymerase that is involved in leading strand DNA replication in eukaryotes. DNA Pol ε in yeast consists of four subunits, Pol2, Dpb2, Dpb3, and Dpb4. Pol2 is the catalytic subunit and Dpb2, Dpb3, and Dpb4 are the accessory subunits. LÄS MER

  3. 2. Oxidation of 1,2-Diols Using Alcohol Dehydrogenases : From Kinetic Characterization to Directed Evolution

    Författare :Cecilia Blikstad; Mikael Widersten; Stefan Lutz; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; enzyme kinetics; alcohol dehydrogenase; directed evolution; enzyme engineering; diol; α-hydroxy aldehyde; Biokemi; Biochemistry;

    Sammanfattning : The use of enzymes as catalysts for chemical transformations has emerged as a “greener” alternative to traditional organic synthesis. An issue to solve though, is that enzymes are designed by nature to catalyze reactions in a living cell and therefore, in many cases, do not meet the requirements of a suitable biocatalyst. LÄS MER

  4. 3. The kinetic mechanism of microsomal glutathione transferase 1 (MGST1)

    Författare :Richard Svensson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Glutathione; glutathione transferase; enzyme mechanism; pre-steady state kinetics; steady state kinetics; membrane protein; xenobiotic metabolism.;

    Sammanfattning : Microsomal glutathione transferase I (MGST1) is a homotrimeric membrane-bound enzyme involved in detoxication of numerous toxic electrophilic compounds, e.g. halogenated alkenes and arenes, alpha, beta-unsaturated hydrocarbons and peroxides. MGST1 is expressed in large amounts in most organs, which speaks for an important role for this enzyme. LÄS MER

  5. 4. Microsomal prostaglandin E synthase 1 and microsomal glutathione transferase 1 : inhibition and mechanism

    Författare :Linda Spahiu; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The Membrane-Associated Proteins in Eicosanoid and Glutathione Metabolism (MAPEG) is a family of six membrane bound proteins with different biological functions. MAPEG superfamily members enable and catalyze reactions where reactive lipid intermediates are either transformed to physiological messengers or turned into unreactive compounds. LÄS MER

  7. 5. Mechanism of enzymes involved in leukotriene C4 biosynthesis

    Författare :H R Shabbir Ahmad; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Cysteinyl leukotrienes (cys-LTs) are potent proinflammatory mediators associated with various diseases including asthma and allergic rhinitis. Leukotriene C4 synthase (LTC4S) and microsomal glutathione transferase 2 (MGST2) catalyze conjugation of the epoxide intermediate LTA4 with GSH to form LTC4, the parent compound of the cys-LTs. LÄS MER