Sökning: "polyclonal antibodies"

Visar resultat 1 - 5 av 88 avhandlingar innehållade orden polyclonal antibodies.

  1. 1. Validation of antibodies for protein profiling : A study using immunohistochemistry on tissue microarrays

    Författare :Linda Paavilainen; Fredrik Pontén; Ola Nilsson; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; antibody validation; monospecific antibodies; protein profiling; immunohistochemistry; tissue microarrays; western blot; fixatives; Pathology; Patologi; Pathology; patologi;

    Sammanfattning : The field of proteomics has rapidly expanded due to the completion of the human genome sequence. This thesis validates affinity-purified monospecific antibodies of polyclonal origin, for protein profiling in a broad spectrum of normal tissues and cells. LÄS MER

  3. 2. Structural investigation of SOD1 aggregates in ALS : identification of prion strains using anti-peptide antibodies

    Författare :Johan Bergh; Thomas Brännström; Stefan L. Marklund; Peter M. Andersen; Joakim Bergström; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ALS; SOD1; prion; motor neuron disease; neurodegeneration; strain; seeding; protein aggregation; transgenic mice; peptide antibodies; Neurology; neurologi;

    Sammanfattning : Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative syndrome characterized by progressive degeneration of motor neurons that result in muscle wasting. The symptoms advance gradually to paralysis and eventually death. Most patients suffer from sporadic ALS (sALS) but 10% report a familial predisposition. LÄS MER

  4. 3. Computational analyses of biological sequences -applications to antibody-based proteomics and gene family characterization

    Författare :Mats Lindskog; Mathias Uhlén; Fredrik Sterky; Andersson Björn; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; antigen; antibodies; antibody-based proteomics; biocomputing; bioinformatics; Bioinformatics; Bioinformatik;

    Sammanfattning : Following the completion of the human genome sequence, post-genomic efforts have shifted the focus towards the analysis of the encoded proteome. Several different systematic proteomics approaches have emerged, for instance, antibody-based proteomics initiatives, where antibodies are used to functionally explore the human proteome. LÄS MER

  5. 4. Transcriptome and Proteome Analysis using Signature Tags

    Författare :Charlotta Agaton; KTH; []
    Nyckelord :functional genomics; 3´-end signature tags; pyrosequencing; amplification; PrEST; chromosome 21; polyclonal antibodies; dual expression; affinity purification;

    Sammanfattning : With the full sequence of the human genome now available, anexciting era in biomedical research has started. The sequenceprovides information about all our genes and greatly increasesthe scope to compare genetic activities in different cells, toanalyze genetic variation between individuals and betweendifferent species and, most importantly, to investigatesystematically the whole genome in a gene-by-gene manner, andthus increase our understanding of gene function. LÄS MER

  7. 5. Perspectives on the Biological Role of Human Prostasomes

    Författare :Lena Carlsson; Outi Hovatta; Uppsala universitet; []
    Nyckelord :Medical sciences; Prostasomes; cryopreservation; human spermatozoa; sperm motility; anti- prostasome antibodies; PC-3 cells; Bacillus megaterium; antibacterial; antisperm antibodies; complement activation; sperm agglutination; infertility; MEDICIN OCH VÅRD; MEDICINE; MEDICIN; Clinical Chemistry; klinisk kemi;

    Sammanfattning : Prostasomes are extracellularly occurring organelles which are secreted in human semen by the prostate gland. Prostasomes have several known biological activities, but their physiological function is still unclear. In this thesis some new aspects were studied on the biological role of the prostasomes. LÄS MER