Sökning: "plasmid DNA"

Visar resultat 6 - 10 av 116 avhandlingar innehållade orden plasmid DNA.

  1. 6. Repair of DNA double-stranded breaks in human cells

    Författare :Susanne Nyström; Göteborgs universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA-PK; DNA double-stranded break; DSB; DNA single-stranded break; SSB; DNA damage; ionizing radiation; IR; DNA repair; calicheamicin gamma1; bleomycin; etoposide; topoisomerase II; radiotherapy; radiosensitizer; ATM;

    Sammanfattning : DNA is continuously subjected to degradation. Therefore, our cells need to constantly repair its DNA to prevent mutations and in the long run cancer. In mammalian cells, when DNA is broken right off, a double-stranded break (DSB) is produced, and the ends are ligated by a process called non-homologous end-joining (NHEJ). LÄS MER

  3. 7. Studies in DNA immunization

    Författare :Cecilia Svanholm; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Chlamydia pneumoniae; DNA vaccine; GM-CSF; HIV-1; hsp60; Nef; Rev; Tat; signal sequence;

    Sammanfattning : This thesis describes investigations and developments of DNA immunization techniques, which were applied in the study of two different pathogens; HIV-1 and Chlamydia pneumoniae. DNA vaccines, i.e. naked plasmid DNA encoding an antigen, represent a novel strategy for generating protective immunity against several infectious diseases. LÄS MER

  4. 8. Recognition and signaling of DNA double-strand breaks in human cells

    Författare :Ismail Hassan Ismail; Göteborgs universitet; []
    Nyckelord :DNA-PK; DNA double-strand break; DSB; DNA single-strand break; SSB; DNA damage; ionizing radiation; IR; DNA repair; calicheamicin kappa1; ATM; hydrogen peroxide; H2AX; radiotherapy; radiosensitizer; flow cytometry;

    Sammanfattning : DNA double-strand breaks (DSBs) are the most toxic type of DNA damage. Fortunately, our cells have highly conserved pathways that detect and repair DSBs. Defects in DSB signaling and DSB repair pathways result in genomic instability that predisposes for cancer development. LÄS MER

  5. 9. Induction of t-cell responses against PSA by plasmid DNA immunization

    Författare :Maxim Pavlenko; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Prostate-specific antigen; DNA vaccine; cytotoxic T lymphocytes;

    Sammanfattning : This thesis is focused on using plasmid DNA encoding human prostate-specific antigen (PSA) for immunotherapy of prostate cancer. PSA is a secreted serine protease, which is produced almost exclusively by prostate epithelial cells. LÄS MER

  7. 10. Integration of Aqueous Two-Phase Systems into Recovery Processes for Biomolecules

    Författare :Cecilia Kepka; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteins; proteins; plasmid DNA; large-scale; protein purification; integration; Expression; aqueous two-phase systems; enzymology; Proteiner; enzymologi;

    Sammanfattning : The main scope of this thesis has been to study and develop new purification methods and also to further develop existing methods for purification to enhance their efficacy. Important parameters in the development of the methods are that they should be suitable for scale-up and possible to be integrated into recovery processes. LÄS MER