Sökning: "plasmid DNA"

Visar resultat 11 - 15 av 116 avhandlingar innehållade orden plasmid DNA.

1. 11. Optical Mapping of Bacterial Plasmids

   Författare :Vilhelm Müller; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; competitive binding; optical mapping; fluorescence microscopy; DNA; plasmids; CRISPR Cas9; antibiotic resistance; nanofluidics; single-molecule;

   Sammanfattning : Bacteria that have acquired resistance to antibiotics represent one of the largest threats to human health and modern health care. The genes encoding resistance are frequently spread via the transfer of plasmids, which are circular double stranded DNA molecules separate from the chromosomal DNA of the bacteria. LÄS MER

3. 12. Purification Processes for Complex Biomacromolecules

   Författare :Hans Blom; Helena Grennberg; Mats Lundgren; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Chromatography; enzymes; mucins; fractionation; plasmid DNA; analysis; clarification; process design; Chemistry with specialization in Surface Biotechnology; Kemi med inriktning mot ytbioteknik;

   Sammanfattning : This thesis details various techniques and considerations for the purification of complex biomacromolecules. Initially an α-mannosidase from babaco fruit was purified using anion exchange-, lectin affinity- and size exclusion chromatography. LÄS MER

4. 13. Production and delivery of recombinant subunit vaccines

   Författare :Christin Andersson; KTH; []
   Nyckelord :Affibody; Affinity chromatography; Affinity purification; DNA immunization; Expression plasmid; Fusion protein; Hydrophobic tag; Iscoms; Lipid tagging; Malaria; Mammalian cell expression; Recombinant immunogen; Respiratory Syncytial Virus; Semliki Fo;

   Sammanfattning : Recombinant strategies are today dominating in thedevelopment of modern subunit vaccines. This thesis describesstrategies for the production and recovery of protein subunitimmunogens, and how genetic design of the expression vectorscan be used to adapt the immunogens for incorporation intoadjuvant systems. LÄS MER

5. 14. Primary Recovery of Biologically Active Compounds Using Macroporous Monolithic Hydrogels

   Författare :Amro Hanora; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Downstream processing; macroporous; Biologically active compounds; Biotechnology; Läkemedelsteknik och relaterad teknik; Pharmaceutical and related technologies; Polymerteknik; biopolymers; Biokemisk teknik; Polymer technology; Makromolekylär kemi; Biochemical technology; Affinity chromatography; Ion exchange chromatography; Expanded bed adsorption chromatography.; Macromolecular chemistry; High through put screening; lactica acids; Endotoxin; Plasmid DNA; Cryogel columns; Bioteknik;

   Sammanfattning : The completion of sequencing of human and other mammalian genomes opens a new era for drug development. New approaches have been proposed for tackling diseases such as development of small molecule therapies based on understanding of human genome and proteome, new proteinous based therapies, where the therapeutic protein can inhibit or modify a certain metabolic pathway or DNA-based therapies delivering a gene into the cell (e. LÄS MER

7. 15. Amelioration of Horizontally Transferred Genes

   Författare :Torbjörn Karfunkel; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; transposoner; horisontell genöverföring; dna; bakterier; plasmider;

   Sammanfattning : The importance of Horizontal (Lateral) Gene Transfer has attracted much attention during the last few decades. It is a very important factor in bacterial evolution and ecology, and it has great implications on human life as well. LÄS MER