Sökning: "plasma proteome"

Visar resultat 1 - 5 av 41 avhandlingar innehållade orden plasma proteome.

  1. 1. Multiplexed absolute quantification of blood plasma proteins using targeted proteomics

    Författare :David Kotol; Mathias Uhlén; Edfors Fredrik; Roman Zubarev; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mass spectrometry; targeted proteomics; stable isotope standards; recombinant protein standards; absolute quantification; blood plasma; multiplex analysis; plasma profiling; Biotechnology; Bioteknologi;

    Sammanfattning : Proteins are the molecular building blocks of all living organisms. They are the functioning actors in metabolism and communication, and they give specific architecture and purpose to all tissues. Their abundance is dynamic and can be interpreted and associated to different physiological states or diseases. LÄS MER

  3. 2. Antibody-based bead arrays for high-throughput protein profiling in human plasma and serum

    Författare :Kimi Drobin; Jochen M. Schwenk; Peter Nilsson; Ulrika Qundos; Angela Silveira; Maria Pernemalm; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteomics; affinity proteomics; immunoassay; antibody microarray; suspension bead array; protein profiling; plasma; serum; biomarker discovery; osteoporosis; inflammatory bowel disease; Crohn s disease; ulcerative colitis; Medical Technology; Medicinsk teknologi;

    Sammanfattning : Affinity-based proteomics utilizes affinity binders to detect target proteins in a large-scale manner. This thesis describes a high-throughput method, which enables the search for biomarker candidates in human plasma and serum. LÄS MER

  4. 3. Design of recombinant antibody microarrays for global proteome analysis

    Författare :Johan Ingvarsson; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bioteknik; oncoproteomics; Recombinant antibody microarray; scFv; Biotechnology; plasma proteome analysis;

    Sammanfattning : Antibody-based microarrays are among the new, rapidly emerging technologies within the field of proteomics that has the potential to evolve into a key proteomic research tool, providing novel means to perform early diagnostics, identify biomarkers and gain insights into disease biology. The aim of this thesis, based on five original papers, was to develop antibody microarrays based on human recombinant scFv antibody fragments for large-scale comprehensive proteome analysis. LÄS MER

  5. 4. Blue native polyacrylamide gel electrophoresis - a functional approach to plant plasma membrane proteome studies

    Författare :Jonas Kjell; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; dodecyl maltoside; BN-PAGE; plant plasma membrane; protein complexes;

    Sammanfattning : In this thesis, I have shown that a method called BN-PAGE (a kind of native gel electrophoresis) can be used for studying the protein composition in the plasma membrane of plants. The method enables the study of native (in the functional units) hydrophobic protein complexes, i.e. LÄS MER

  7. 5. Biomarker detection via lab-on-a-chip integrated immunoaffinity approach for fluorescence and mass spectrometry readout

    Författare :Asilah Ahmad Tajudin; Avdelningen för Biomedicinsk teknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; immunoaffinity; affinity prefractionation; immunoMALDI; solid-phase extraction; protein digestion; ISET; antibody microarray; signal amplification; fluorescence; microfluidics; lab on a chip; separation; acoustophoresis; ultrasound; acoustic radiation force; plasma; proteomics; MALDI mass spectrometry; immunoassay; porous silicon.;

    Sammanfattning : The blood/plasma protein biomarker profile represents the pathological and physiological changes relating to human diseases. However, the plasma proteome reflects a high degree of complexity that results in a challenge for most analytical methods in clinical diagnostics. LÄS MER