Sökning: "planar lipid bilayers"

Visar resultat 1 - 5 av 7 avhandlingar innehållade orden planar lipid bilayers.

  1. 1. Lipid Vesicle Fusion: Investigation, Generation and Manipulation of Cell-Membrane Mimics

    Författare :Lisa Simonsson; Chalmers University of Technology; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; diffusion; spreading lipid bilayers; cell mimic; micromanipulation; lipid bilayer; microfluidics; site-specificity; DNA; membrane-receptor interactions; total internal reflection fluorescence microscopy; model system; self-assembly; TIRF microscopy; FRET; exocytosis; vesicles; fluorescence resonance energy transfer; biomembranes; amperometry; membrane fusion;

    Sammanfattning : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. In this work, cell-membrane mimics were constructed and evaluated as models for studies of the membrane-fusion process. LÄS MER

  2. 2. On the Formation of Supported Phospholipid Bilayers

    Författare :Erik Reimhult; Chalmers University of Technology; []
    Nyckelord :vesicle adsorption; formation kinetics; SPR; QCM-D; SPB; AFM; supported lipid bilayer; supported membrane; liposome;

    Sammanfattning : Lipid bilayers are a central component of both the structure and function of biological membranes, like the one surrounding cells of living organisms. Since the lipid bilayer provides a host matrix for membrane proteins, which perform many of the specialized tasks in cells like energy, signal and material transduction, researchers have tried to develop artificial model membranes to study a variety of membrane protein functions. LÄS MER

  3. 3. Thermo- and chemosensitive properties of Transient Receptor Potential Ankyrin 1 ion channels

    Författare :Lavanya Moparthi; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TRP ion channels; TRPA1; ankyrin repeat domain; thermosensation; chemosensation; cold sensor; electrophiles; non-electrophiles; planar lipid bilayers; patch-clamp; fluorescence spectroscopy; mass spectrometry;

    Sammanfattning : The ability to sense and accommodate to an ever-changing environment is crucial for the survival of living organisms. Transient Receptor Potential (TRP) ion channels comprise a large superfamily of cation conducting membrane proteins that function as molecular sensors in diverse sensory processes including perception of light, taste, smell, sound, touch and temperature. LÄS MER

  4. 4. Waveguide Evanescent-Field Microscopy for Label-Free Monitoring of Biological Nanoparticles: Fabrication, Characterization and Application

    Författare :Mokhtar Mapar; Chalmers University of Technology; []
    Nyckelord :NATURVETENSKAP; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; NATURAL SCIENCES; Biosensor; Protein interaction; Waveguide scattering microscopy; Waveguide fluorescence microscopy; Supported lipid bilayer; Image processing; Lipid vesicle; Nanofabrication; Exosome;

    Sammanfattning : The recent development of microscopy methods, biological assays and bioanalytical sensors has significantly advanced the understanding of biological systems. Surface-based bioanalytical sensors have in recent years gained increased interest thanks to improvements in sensitivity and simplicity to use. LÄS MER

  5. 5. Supported Lipid Membranes and Their Use for the Characterization of Biological Nanoparticles

    Författare :Silver Jõemetsa; Chalmers University of Technology; []
    Nyckelord :NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; NATURAL SCIENCES; microfluidics; size determination; fluorescence microscopy; lipophilic dyes; supported lipid bilayer; diffusion;

    Sammanfattning : Biological nanoparticles (BNPs) are nano-sized lipid vesicles of biological origin, which are involved in multiple biological processes. BNP characterization techniques are critical for improving the understanding of how these particles contribute to cellular communication, viral infections and drug-delivery applications. LÄS MER