Sökning: "pichia pastoris"

Visar resultat 6 - 10 av 53 avhandlingar innehållade orden pichia pastoris.

1. 6. Expression of Manganese Lipoxygenase and Site-Directed Mutagenesis of Catalytically Important Amino Acids : Studies on Fatty Acid Dioxygenases

   Författare :Mirela Cristea; Ernst H. Oliw; Chao Su; Olof Rådmark; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Pharmaceutical pharmacology; Dioxygenase; Gaeumannomyces graminis; lipoxygenase; Magnaporthe grisea; oxylipin; Pichia pastoris; hydroperoxide isomerase; polyunsaturated fatty acids; Farmaceutisk farmakologi; Pharmaceutical pharmacology; Farmaceutisk farmakologi;

   Sammanfattning : Polyunsaturated fatty acids can be bioactivated by two families of dioxygenases, which either contain non-heme iron (lipoxygenases) or heme (cyclooxygenases, linoleate diol synthases and α-dioxygenases).Lipoxygenases and their products play important roles in the pathophysiology of plants and fungi. LÄS MER

3. 7. Production and engineering of a xyloglucan endo-transglycosylase from Populus tremula x tremuloides

   Författare :Maria Henriksson; Tuula Teeri; KTH; []
   Nyckelord :LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES; xyloglucan endo-transglycosylase; retaining glycoside hydrolase; glycosynthase; Pichia pastoris; fed-batch fermentation; expanded-bed adsorption; Plant biotechnology; Växtbioteknik;

   Sammanfattning : The aim of this work was to develop a production process for the enzyme xyloglucan endo-transglycosylase from Populus tremula x tremuloides (PttXET16-34). The natural transglycosylating activity of this enzyme has previously been employed in a XET-Technology. LÄS MER

4. 8. In vitro and in vivo approaches in the characterization of XTH gene products

   Författare :Nomchit Kaewthai; Harry Brumer; Jocelyn Rose; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Arabiodopsis thaliana; Hordeum vulgare; plant cell wall; xyloglucan; glycoside hydrolase family 16; xyloglucan endo-transglycosylase hydrolase gene family; xyloglucan endo-hydrolase; heterologous protein expression; Pichia pastoris; T-DNA insertion; in situ XET XEH assay; high-througput screening; Plant physiology; Växtfysiologi; Biochemistry; Biokemi; Functional genomics; Funktionsgenomik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : ABSTRACT The xyloglucan endo-transglycosylase/hydrolase (XTH) genes are found in all vascular and some nonvascular plants. The XTH genes encode proteins which comprise a subfamily of glycoside hydrolase (GH) family 16 in the Carbohydrate-Active enZYmes (CAZY) classification. LÄS MER

5. 9. Design of enzymatic systems for the modification of cellulose fiber surfaces

   Författare :Malin Gustavsson; KTH; []
   Nyckelord :Candida antarctica lipase B; Pichia pastoris; cellulose-binding module; proteolysis; carbohydrate ester; xyloglucan endotransglycosylase; fiber modification;

   Sammanfattning : This thesis describes the design of enzymatic systems forthe modification of cellulose fiber surfaces. A single-enzymeapproach employed a conjugate between theNeocallimastix patriciarumCel6A cellulose-bindingmodule and lipase B fromCandida antarctica(CBM-CALB) to enhance theaccessibility of the enzyme to the target hydroxyl groups. LÄS MER

7. 10. Functional studies of a membrane-anchored cellulase from poplar

   Författare :Ulla Jonsson Rudsander; Tuula Teeri; Eva Nordberg-Karlsson; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Populus; cellulose biosynthesis; endo-1; 4-b-glucanase; cellulase; Pichia pastoris; Arabidopsis thaliana; NMR spectroscopy; cleavage pattern; stereochemistry; transglycosylation; complementation; hybrid proteins; overexpression; substrate analysis; overglycosylation; Bioengineering; Bioteknik;

   Sammanfattning : Cellulose in particular and wood in general are valuable biomaterials for humanity, and cellulose is now also in the spotlight as a starting material for the production of biofuel. Understanding the processes of wood formation and cellulose biosynthesis could therefore be rewarding, and genomics and proteomics approaches have been initiated to learn more about wood biology. LÄS MER