Sökning: "phage display"
Visar resultat 6 - 10 av 97 avhandlingar innehållade orden phage display.
6. Molecular Recognition in Antibody Engineering. Studies on recombinant and phage displayed antibodies
Sammanfattning : In vitro generation of antibody fragments of desired specificity and affinity plays an important role since it permits the generation of reagents which may be valuable for diagnostic and therapeutic applications. Furthermore, the kinetic parameters for an antibody-antigen interaction, rather than the affinity, has shown to correlate with biological functions like virus neutralization. LÄS MER
7. Using peptide-phage display to capture conditional motif-based interactions
Sammanfattning : This thesis explores the world of conditional protein-protein interactions using combinatorial peptide-phage display and proteomic peptide-phage display (ProP-PD). Large parts of proteins in the human proteome do not fold in to well-defined structures instead they are intrinsically disordered. LÄS MER
8. Protein–DNA Recognition : In Vitro Evolution and Characterization of DNA-Binding Proteins
Sammanfattning : DNA-recognizing proteins are involved in a multitude of important life-processes. Therefore, it is of great interest to understand the underlying mechanisms that set the rules for sequence specific protein–DNA interactions. Previous attempts aiming to resolve these interactions have been focused on naturally occurring systems. LÄS MER
9. Interaction engineered three-helix bundle domains for protein recovery and detection
Sammanfattning : HTML clipboard The great advances in DNA technology, e.g. sequencing and recombinant DNA techniques, have given us the genetic information and the tools needed to effectively produce recombinant proteins. Recombinant proteins are valuable means in biotechnological applications and are also emerging as alternatives in therapeutic applications. LÄS MER
10. Engineering strategies for ABD-derived affinity proteins for therapeutic and diagnostic applications
Sammanfattning : Small stable protein domains are attractive scaffolds for engineering affinity proteins due to their high tolerance to mutagenesis without loosing structural integrity. The albuminbinding domain is a 5 kDa three-helix bundle derived from the bacterial receptor Protein G with low-nanomolar affinity to albumin. LÄS MER