Sökning: "phage display"

Visar resultat 11 - 15 av 97 avhandlingar innehållade orden phage display.

  1. 11. An albumin-binding domain as a scaffold for bispecific affinity proteins

    Författare :Johan Nilvebrant; Sophia Hober; Andrew Bradbury; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; albumin-binding domain; bispecific; albumin; affinity protein; phage display; staphylococcal display; orthogonal affinity purification; TNF-alpha; ErbB2; ErbB3; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : Protein engineering and in vitro selection systems are powerful methods to generate binding proteins. In nature, antibodies are the primary affinity proteins and their usefulness has led to a widespread use both in basic and applied research. LÄS MER

  3. 12. Bacterial display systems for engineering of affinity proteins

    Författare :Filippa Fleetwood; John Löfblom; Harald Kolmar; KTH; []
    Nyckelord :Combinatorial protein engineering; staphylococcal display; Affibody; biparatopic; VEGFR2; nanobody; E. coli display; autotransporter; Bioteknologi; Biotechnology;

    Sammanfattning : Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. LÄS MER

  4. 13. Functional studies and engineering of family 1 carbohydrate-binding modules

    Författare :Janne Lehtiö; KTH; []
    Nyckelord :Cellulose-binding; carbohydrate-binding modules; phage display; bacterial surface display; combinatorial protein library; protein engineering; Trichoderma reesei; Staphylococcus carnosus;

    Sammanfattning : The family 1 cellulose-binding modules (CBM1) form a groupof small, stable carbohydrate-binding proteins. These modulesare essential for fungal cellulosedegradation. This thesisdescribes both functional studies of the CBM1s as well asprotein engineering of the modules for several objectives. LÄS MER

  5. 14. Phenotypic antibody discovery and mining of complex antibody libraries

    Författare :Anne Ljungars; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Antibody discovery; Phage display; Phenotypic screening; Cell selection; Deep mining; Complex phage pools;

    Sammanfattning : Therapeutic antibodies are the fastest growing class of drugs but suffer from a crowded target-space where many antibodies, against a few targets, are developed in parallel. The aim of this thesis is to find methods to enable discovery of novel antibody-target combinations and thereby increase the target-space. LÄS MER

  7. 15. Biomolecular recognition : recombinant antibodies, phage display and eukaryotic cloning systems

    Författare :Michelle A. Alting-Mees; Uppsala universitet; []
    Nyckelord :MEDICINE; MEDICIN;

    Sammanfattning : .... LÄS MER