Sökning: "phage display selection"

Visar resultat 1 - 5 av 43 avhandlingar innehållade orden phage display selection.

  1. 1. Selection and design in antibody phage display

    Författare :Jonas Persson; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; specificity fine-tuning.; phage display; MUC1; automated selection; antigenicity; antibody; affinity maturation;

    Sammanfattning : The immune system is an important part of the physiology of higher organisms. In the immune system antibodies play a key role in neutralizing and clearing potentially lethal threats from the environment. The importance of antibodies in the immune system has made them come to the attention as a tool in chemistry and medicine. LÄS MER

  3. 2. Molecular Recognition in Antibody Engineering. Studies on recombinant and phage displayed antibodies

    Författare :Ann-Christin Malmborg Hager; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; transplantation; affinity; antibody; kinetic; Immunologi; serologi; serology; Immunology; Bioteknik; Biotechnology; library; selection; surface display; phage display; association rate; dissociation rate;

    Sammanfattning : In vitro generation of antibody fragments of desired specificity and affinity plays an important role since it permits the generation of reagents which may be valuable for diagnostic and therapeutic applications. Furthermore, the kinetic parameters for an antibody-antigen interaction, rather than the affinity, has shown to correlate with biological functions like virus neutralization. LÄS MER

  4. 3. Ribosome display for selection and evolution of affibody molecules

    Författare :Sebastian Grimm; Per-Åke Nygren; Mike Taussig; KTH; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; affibody; ribosome display; phage display; combinatorial protein engineering; library; murine IgG1; SATB1; Ras; Raf; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

    Sammanfattning : Affinity proteins are invaluable tools in biotechnological and medical applications. This thesis is about combinatorial protein engineering principles for the generation of novel affinity proteins to purify mouse immunoglobulin, detect a potential cancer marker protein or inhibit a cell proliferation pathway. LÄS MER

  5. 4. Selection and use of affinity proteins developed by combinatorial engineering

    Författare :Kristofer Sandström; KTH; []
    Nyckelord :phage display; combinatorial; selection; cd28; cllulosome; cellulose;

    Sammanfattning : In affinity protein biotechnology the selective bindingbetween a chosen protein and an interacting biomolecule isutilized for a variety of applications including bioseparation,detection and therapy. Traditionally, affinity proteinsrecruited for such applications have been derived from naturalproteins or immunoglobulins generated via immunization routes. LÄS MER

  7. 5. The biology of filamentous phage infection - implications for display technology

    Författare :Fredrik Karlsson; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biotechnology; Bioteknik; infection mechanism; selective infection; TolA; Escherichia coli; pIII; Filamentous phage; Phage display technology;

    Sammanfattning : Phage display technology is a biotechnological tool that can be used to obtain molecules/reagents able to discriminate between target molecules. Reagents with such features can be used in diverse settings like biological chemistry, diagnostics and in therapeutic applications. LÄS MER