Sökning: "peptide-phage display"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden peptide-phage display.

1. 1. Using peptide-phage display to capture conditional motif-based interactions

   Författare :Gustav Sundell; Ylva Ivarsson; Per Jemth; Attila Reményi; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Protein-protein interaction; PDZ domain; phosphorylation; peptide-phage display; ProP-PD; phospho-peptide; phosphomimetic substitution; Scribble; NMR; DLG1; PTM; affiinity; Biokemi; Biochemistry;

   Sammanfattning : This thesis explores the world of conditional protein-protein interactions using combinatorial peptide-phage display and proteomic peptide-phage display (ProP-PD). Large parts of proteins in the human proteome do not fold in to well-defined structures instead they are intrinsically disordered. LÄS MER

3. 2. Identification of SLiMs: Mapping and characterizing motif-based protein interactions

   Författare :Muhammad Ali; Ylva Ivarsson; Shoshana Wodak; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Phage display; protein-protein interactions; short linear motifs; PDZ; FERM; affinity; x-ray crystallization; molecular docking; NLS;

   Sammanfattning : During the last twenty years it has become evident that about 35-40% of amino acids in the proteome are in regions that have evolved to remain unstructured. These intrinsically disordered regions contain short linear motifs (SLiMs), which serve as docking sites for protein-protein interactions. LÄS MER

4. 3. Evolution and viral mimicry of short linear motif-mediated interactions

   Författare :Filip Mihalič; Per Jemth; Leo James; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; phage display; protein-protein interactions; large-scale discovery; short linear motifs; viral mimcry; viral hijacking; Biokemi; Biochemistry; Medical Biochemistry; Medicinsk biokemi;

   Sammanfattning : Proteins are one of the most fundamental building blocks of life and their interactions regulate every cellular process. Historically they have been conceptualized as predominantly folded entities with well-defined secondary and tertiary structures. LÄS MER

5. 4. Bacteriophages and cryogels: A new efficient tool in bioseparation

   Författare :Wim Noppe; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biopanning; Cryogel; Phage Display Technology; Chromato-panning;

   Sammanfattning : Abstract Due to developments in the last decades in the field of protein production, new technologies for bio-separation have evolved. Especially in the field of affinity chromatography, the search for new ligands is of great interest. Besides the antibodies used as affinity ligands, synthetic ligands were introduced. LÄS MER

7. 5. Diving into short linear motifs : Large-scale identification of endogenous and host-pathogen protein-protein interactions and further characterized by deep mutational scanning

   Författare :Caroline Benz; Ylva Ivarsson; Birthe B. Kragelund; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; protein-protein interactions; short linear motif; endogenous interactions; host-pathogen; viral hijacking; deep mutational scanning; Biokemi; Biochemistry;

   Sammanfattning : Short linear motifs (SLiMs) are protein-protein interaction sites that play an essential role in distinct cellular processes. Those interactions are challenging to capture by common high-throughput methods. LÄS MER