Sökning: "p2 integrase"

Visar resultat 1 - 5 av 7 avhandlingar innehållade orden p2 integrase.

  1. 1. Structure-Function Studies of Bacteriophage P2 Integrase and Cox protein

    Författare :Jesper Eriksson; Elisabeth Haggård; Karin Hammer; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Bacteriophage P2; site-specific recombination; integrase; transcriptional switch; Genetics; Genetik;

    Sammanfattning : Probably no group of organisms has been as important as bacteriophages when it comes to the understanding of fundamental biological processes like transcriptional control, DNA replication, site-specific recombination, e.t.c.The work presented in this thesis is a contribution towards the complete understanding of these organisms. LÄS MER

  3. 2. Site-specific recombination of P2-like phages; possible tools for safe gene therapy : A focus on phage ΦD145

    Författare :Sridhar Mandali; Elisabeth Haggård-Ljungquist; Richard Calendar; Stockholms universitet; []
    Nyckelord :bacteriophage; integrase; site-specific recombination; NATURAL SCIENCES; NATURVETENSKAP; molekylärgenetik; Molecular Genetics;

    Sammanfattning : P2-like bacteriophages integrate their genome into the E. coli host cell by a site-specific recombination event upon lysogenization. The integrative recombination occurs between a specific sequence in the phage genome, attP, and a specific sequence in the host genome, attB, generating the host-phage junctions attL and attR. LÄS MER

  4. 3. Site-specific recombination in P2-like coliphages

    Författare :Carlos Cardoso Palacios; Elisabeth Haggård-Ljungquist; Didier Mazel; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Genetics; Genetik; molekylärgenetik; Molecular Genetics;

    Sammanfattning : The scope of these studies has been to investigate the site-specific recombination systems of P2-like coliphages, both in an evolutionary perspective by a comparative analysis of related phages as well as in a functional perspective. Surveys of P2-like phages in Escherichia coli isolated from nature reveal the existence of seven discrete immunity classes and three integration sites, one of them previously unknown. LÄS MER

  5. 4. Site-Specific Recombination : Integrases, Accessory Factors and DNA Targets of P2-like Coliphages

    Författare :Lina Sylwan; Elisabeth Haggård-Ljungquist; Margaret C. M. Smith; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Genetics; Genetik; molekylärgenetik; Molecular Genetics;

    Sammanfattning : The temperate coliphage P2 and its family members integrate their genomes into the host Escherichia coli chromosome by a site-specific recombination mechanism to form lysogeny. Integration takes place between the complex phage attP site and the simple bacterial attB site and is catalyzed by the phage encoded integrase (Int). LÄS MER

  7. 5. Functional characterization of a phage integrase and its possible use in gene therapy

    Författare :Clara Frumerie; Elisabeth Haggård-Ljungquist; Lars Sundström; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Clinical genetics; Klinisk genetik;

    Sammanfattning : Bacteriophage P2 infecting Escherichia coli can integrate into the bacterial chromosome by site-specific recombination, which is catalyzed by the P2 Int recombinase. The recombination event takes place between the phage attachment site, attP, and the bacterial attachment site, attB. LÄS MER