Sökning: "optical waveguide microscopy"

Visar resultat 1 - 5 av 8 avhandlingar innehållade orden optical waveguide microscopy.

  1. 1. Waveguide Evanescent-Field Microscopy for Label-Free Monitoring of Biological Nanoparticles: Fabrication, Characterization and Application

    Författare :Mokhtar Mapar; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Biosensor; Protein interaction; Waveguide scattering microscopy; Waveguide fluorescence microscopy; Supported lipid bilayer; Image processing; Lipid vesicle; Nanofabrication; Exosome;

    Sammanfattning : The recent development of microscopy methods, biological assays and bioanalytical sensors has significantly advanced the understanding of biological systems. Surface-based bioanalytical sensors have in recent years gained increased interest thanks to improvements in sensitivity and simplicity to use. LÄS MER

  3. 2. Physical characterization of exosomes and other extracellular vesicles using surface-based sensing

    Författare :Deborah Rupert; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; nanoparticle tracking analysis; exosomes; scattering; surface-based biosensing; optical density; cluster of differentiation 63 CD63 ; liposomes; label-free; fluorescence; surface plasmon resonance; Extracellular vesicles; specificity; antibody; lipid content; single particle; quantification; size distribution; optical waveguide microscopy;

    Sammanfattning : Extracellular vesicles (EVs) are hundred of nanometer sized particles secreted by almost all cell types. Exosomes are one type of EVs today intensively investigated due to the increased evidence suggesting that they play important roles as communicating vehicles in a broad range of physiological processes, pathological conditions and disease diagnostics. LÄS MER

  4. 3. Multiparametric Optical Characterization of Biological Nanoparticles using Evanescent Field Sensing

    Författare :Mattias Sjöberg; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein corona; single-particle; liposomes; SPR; lipid nanoparticles; Waveguide scattering microscopy; label-free; mass quantification.;

    Sammanfattning : In light of the increasingly realized dependence of many biological functions on nanoscopic supramolecular assemblies, also including novel biotechnological applications, there is a need for advanced analysis methods capable of accurately quantifying different characteristics of these elusive entities. The prime aim of this thesis is the development and utilization of surface-based bioanalytical sensing methods for quantitative characterization of biological nanoparticles. LÄS MER

  5. 4. Lightguiding of Fluorescence in Nanowires : Principles, Optimization and Implementation for Biosensing

    Författare :Damiano Verardo; NanoLund: Centre for Nanoscience; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Nanowires; Biosensing; Fluorescence microscopy;

    Sammanfattning : Due to their unique optical properties, III-V nanowires are promising candidates for the development of efficient solar cells, LEDs and nanoscaled lasers. The interaction of nanowires with fluorescent molecules is also of interest, as these nanostructures can act as nanoscaled optical fibers. LÄS MER

  7. 5. Liposomes for mucosal vaccine delivery: physicochemical characterization and biological application

    Författare :Karin Norling; Chalmers tekniska högskola; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; nanoparticles; vaccine carriers; antigen presentation; dendritic cells; surface plasmon resonance; flow cytometry; liposomes; cellular uptake; fluorescence microscopy; influenza;

    Sammanfattning : Liposomes are attractive vaccine carriers due to their potential to act as adjuvants, and to the fact that their composition and characteristics are virtually endlessly customizable. However, the precise physicochemical profile of an ideal carrier liposome for mucosal vaccines is still widely unknown, and how different properties affect key steps in the acquisition of protective immunity remains to be elucidated. LÄS MER