Sökning: "open microchannel"

Hittade 5 avhandlingar innehållade orden open microchannel.

  1. 1. Sample preconcentration in open microchannels : Combinations with MALDI and nano-ESI mass spectrometry and computer simulations

    Författare :Saara Mikkonen; Åsa Emmer; Leopold Ilag; Jenny Ottosson Takanen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Preconcentration; open microchannel; microchip; MALDI; electrospray ionization; nanoelectrospray; mass spectrometry; computer simulation;

    Sammanfattning : In this thesis a novel concept for preconcentration of biomolecules in open microchannels is presented. The preconcentration is based on electromigration of charged analytes, and detection is performed with matrix-assisted laser desorption/ionization (MALDI) or nano-electrospray ionization (nESI) mass spectrometry (MS). LÄS MER

  3. 2. Electrophoretic focusing in microchannels combined with mass spectrometry : Applications on amyloid beta peptides

    Författare :Saara Mikkonen; Åsa Emmer; Johan Jacksén; Tapio Kotiaho; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; amyloid beta; computer simulation; electrophoresis; electrospray ionization; isoelectric focusing; isotachophoresis; MALDI; mass spectrometry; microchannel; microchip; nano-electrospray ionization; preconcentration; separation; Kemi; Chemistry;

    Sammanfattning : Analysis of low-abundance components in small samples remains a challenge within bioanalytical chemistry, and new techniques for sample pretreatments followed by sensitive and informative detection are required. In this thesis, procedures for preconcentration and separation of proteins and peptides in open microchannels fabricated on silicon microchips are presented. LÄS MER

  4. 3. Improved Techniques for Protein Analysis Focusing on Membrane Proteins and Hydrophobic Peptides

    Författare :Theres Redeby; Åsa Emmer; Jonas ergquist; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Integral membrane proteins; Hydrophobic peptides; Sample handling; MALDI-TOF-MS; Structured sample support; CE; fraction collection; microchannel; Analytical chemistry; Analytisk kemi;

    Sammanfattning : In this thesis, the vital cell functions performed by integral membrane proteins (IMPs) are briefly discussed. Such proteins are under-represented in most protein studies due to the hydrophobic nature of IMPs, which seriously complicate their solubilization, sample handling, preparation, separation and analysis. LÄS MER

  5. 4. Beta Camera: Development and applications

    Författare :Kaj Ljunggren; Lund Medicinsk strålningsfysik; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Klinisk fysiologi; radiologi; tomografi; medicinsk instrumentering; medical instrumentation; tomography; radiology; Clinical physics; microchannel plate; radionuclides; scintillator; autoradiography; Beta Camera;

    Sammanfattning : A new detector for on-line digital detection of charged particles (beta-particles, conversion electrons and alpha-particles) from thin samples for rapid autoradiography has been developed; the Beta Camera. The device consists of a photon-sensitive detector (image photon detector, IPD); the main component of which is a microchannel plate. LÄS MER

  7. 5. High-Pressure Microfluidics

    Författare :Sam Ogden; Klas Hjort; Greger Thornell; Wouter van der Wijngaart; Uppsala universitet; []
    Nyckelord :phase change; actuator; valve; pump; supercritical fluid; Teknisk fysik med inriktning mot mikrosystemteknik; Engineering Science with specialization in Microsystems Technology;

    Sammanfattning : In this thesis, some fundamentals and possible applications of high-pressure microfluidics have been explored. Furthermore, handling fluids at high pressures has been addressed, specifically by creating and characterizing strong microvalves and pumps.A variety of microstructuring techniques was used to realize these microfluidic devices, e.g. LÄS MER