Sökning: "oligonucleotide DNA probes"

Visar resultat 1 - 5 av 23 avhandlingar innehållade orden oligonucleotide DNA probes.

  1. 1. Padlock probes : Circularizing oligonucleotides for localized detection of DNA sequence variants

    Författare :Mats Nilsson; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

    Sammanfattning : Present techniques for localized detection of DNA sequence variants have limitations due to problems with sensitivity, specificity, signal localization or ability to perform many analyses simultaneously.In this study a novel gene analytic reagent is presented - the padlock probe. LÄS MER

  3. 2. Selector Technology : For Multiplex DNA Analysis

    Författare :Fredrik Dahl; Mats Nilsson; Ulf Landegren; Ivo Glynne Gut; Uppsala universitet; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genetic engineering; DNA technology; Genteknik; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik;

    Sammanfattning : A majority of methods for identifying sequences in the human genome involve target sequence amplification through PCR. This work presents novel methods for amplifying circularized DNA and presents solutions for some major limitations of PCR. LÄS MER

  4. 3. Advancing DNA-based proximity methods

    Författare :Mattias Leino; Ola Söderberg; John Löfblom; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; in situ; proximity dependent intiation of hybridization chain reaction; hybridization chain reaction; Oligonucleotide design; proximity ligation assay; protein interaction; microscopy; purification; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

    Sammanfattning : Cellular functions are governed by intricate chains of interactions between proteins. In order to properly understand cellular biology one must look into, not only protein function, but also its interacting network. LÄS MER

  5. 4. Novel Methods for Synthesis of High Quality Oligonucleotides

    Författare :Andrey Semenyuk; Marek Kwiatkowski; Ulf Landegren; Joachim W. Engels; Uppsala universitet; []
    Nyckelord :Organic chemistry; oligonucleotide; solid-phase synthesis; RNA synthesis; phosphoramidite; abasic sites; depurination; 2 -O-DTM; protecting group; cartridge; conjugate; dithiomethyl linker; base-stable linker; oligonucleotide purification; nucleoside; Organisk kemi;

    Sammanfattning : The first part of the work describes a procedure of oligonucleotide purification using a reversed-phase cartridge. The developed method employs a very efficient yet mild oligonucleotide detritylation on the cartridge support allowing fast purification of oligonucleotides regardless of their 5´-modification. LÄS MER

  7. 5. Molecular analysis of the oral microbiota of dental diseases

    Författare :Eleni Kanasi; Sirkka Asikainen; Anne CR Tanner; Ingegerd Johansson; Gunnar Dahlén; Umeå universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; early childhood caries; early periodontitis; 16S rDNA cloning and sequencing; whole genomic DNA probes; oligonucleotide DNA probes; PCR; diversity; molecular; aggregatibacter actinomycetemcomitans; serotypes; Oral microbiology; Oral mikrobiologi;

    Sammanfattning : Traditionally, bacterial culture has been used for bacterial detection, allowing study of living microorganisms. Molecular methods are rapid and allow simultaneous identification of numerous species and uncultivated phylotypes. LÄS MER