Sökning: "nucleoside transcriptase inhibitor resistance"

Visar resultat 1 - 5 av 12 avhandlingar innehållade orden nucleoside transcriptase inhibitor resistance.

1. 1. Biosensor Studies of Ligand Interactions with Structurally Flexible Enzymes : Applications for Antiviral Drug Development

   Författare :Matthis Geitmann; U. Helena Danielson; Marc H. V. van Regenmortel; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; SPR biosensor; HIV-1 reverse transcriptase; HCMV protease; interaction kinetics; drug discovery; non-nucleoside inhibitor; resistance; Biokemi; Biochemistry; Biokemi;

   Sammanfattning : The use of a surface plasmon biosensor fills a missing link in kinetic studies of enzymes, since it measures directly the interaction between biomolecules and allows determination of parameters that are determined only indirectly in activity assays. The present thesis deals with kinetic and dynamic aspects of ligand binding to two viral enzymes: the human cytomegalovirus (HCMV) protease and the human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). LÄS MER

3. 2. Antiretroviral treatment of HIV-1 in Sweden with focus on virological aspects

   Författare :Erik Sörstedt; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; HIV-1; antiretroviral therapy; transient viremia; viral blip; nucleoside transcriptase inhibitor resistance; dolutegravir; baseline viral load; HIV RNA; virological failure;

   Sammanfattning : From a clinical standpoint, there are many factors to consider when optimizing the care for people living with HIV (PLWH). With help from clinical guidelines, most obstacles can be addressed. Expanded knowledge is however in constant demand, from local conditions to universal processes. LÄS MER

4. 3. Reverse transcriptase assay for analysis of resistance to anti-HIV drugs and their mechanism of action

   Författare :Xingwu Shao; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :HIV-1; RT; antiviral drugs; NRTls; NNRTls; drug resistance; viral load;

   Sammanfattning : Reverse transcriptase (RT) is a viral enzyme and one of the main targets for drugs against human immunodeficiency virus (HIV). This work presents a set of sensitive non-radioactive RT assays were developed: standard RT inhibition assay (IC assay); RT binding inhibition assay; RTprotein detection assay and template-primer destruction assay or chain termination assay (CT assay). LÄS MER

5. 4. Studies of drug resistance in minor HIV quasispecies

   Författare :Halime Ekici; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : The main objective was to study drug resistance mutations (DRM) in the HIV-1 reverse transcriptase (RT) gene of minor HIV-1 quasispecies, not detectable with standard techniques. Sensitive allele-specific PCR (AS- PCR) and next-generation sequencing (NGS) were developed to study resistance to drugs of relevance in low- and middle-income countries (LMIC); the nucleoside analogue RT inhibitor (NRTI) lamivudine (3TC) and the non-nucleoside RT inhibitors (NNRTIs) efavirenz (EFV) and nevirapine (NVP). LÄS MER

7. 5. Towards a New Generation of Anti-HIV Drugs : Interaction Kinetic Analysis of Enzyme Inhibitors Using SPR-biosensors

   Författare :Malin Elinder; U. Helena Danielson; Maurizio Botta; Uppsala universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; drug discovery; fragment; screening; reverse transcriptase; microbicides; interaction analysis; NNRTIs; Biochemistry; Biokemi; Biokemi; Biochemistry;

   Sammanfattning : As of today, there are 25 drugs approved for the treatment of HIV and AIDS. Nevertheless, HIV continues to infect and kill millions of people every year. Despite intensive research efforts, both a vaccine and a cure remain elusive and the long term efficacy of existing drugs is limited by the development of resistant HIV strains. LÄS MER