Sökning: "nucleic acid quantification"

Visar resultat 1 - 5 av 15 avhandlingar innehållade orden nucleic acid quantification.

  1. 1. Nucleic acid analysis tools Novel technologies and biomedical applications

    Detta är en avhandling från Stockholm : Department of Biochemistry and Biophysics, Stockholm University

    Författare :Iván Hernández-Neuta; Stockholms universitet.; [2017]
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Nucleic acid analysis; padlock probes; selector probes; rolling circle amplification; microfluidics; single-molecule detection; digital quantification; fluorescence microscopy; mobile phone microscopy; molecular diagnostics; virology; biokemi; Biochemistry;

    Sammanfattning : Nucleic acids are fundamental molecules of living organisms functioning essentially as the molecular information carriers of life. From how an organism is built to how it responds to external conditions, all of it, can be found in the form of nucleic acid sequences inside every single cell of every life form on earth. LÄS MER

  2. 2. Single Molecule Detection Microfluidic Automation and Digital Quantification

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Malte Kühnemund; Uppsala universitet.; [2016]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

    Sammanfattning : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. LÄS MER

  3. 3. Nucleic acid-based detection and characterization of hepatitis C virus

    Detta är en avhandling från Stockholm : Karolinska Institutet, false

    Författare :Yun Zhibing; Karolinska Institutet.; Karolinska Institutet.; [1995]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; HCV; PCR; genotype; HVRI; heterogeneity; IFN-a; immunodeficiency;

    Sammanfattning : GENERAL Nucleic acid-based methods weredeveloped to detect and characterizethe HCV genome in immunocompe-tent and immunodeficient pahents ofwhom some were IFN-a treated.HCV RNA was found in most sera(95%), livers (97%) and PBMC(100%) of patients with conflrmedchronic hepatitis, but also in sera(21%) of patients with indeterminateconfirmatory antibody test, especial-ly those with c22 antigen reactivity. LÄS MER

  4. 4. Point of care microfluidic tool development for resource limited settings

    Detta är en avhandling från Stockholm : KTH Royal Institute of Technology

    Författare :Indradumna Banerjee; KTH.; [2019]
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Blood; control; cell separation; centrifugal microfluidics; diagnostics; elasto-inertial; hematocrit level; microfluidics; neonatal diagnostics; nucleic acid quantification; point of care; particle focusing; resource limited settings.; Biotechnology; Bioteknologi;

    Sammanfattning : The development of point of care diagnostics using recent advances in microfluidics have the potential to transform health care in several ways, especially in resource limited settings with limited access to advanced health care infrastructure. However, translating a point of care device to reality is often a challenging task because of the complexities involved in integrating a number of diverse engineering concepts into an easy to use, accurate and portable device. LÄS MER

  5. 5. Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays

    Detta är en avhandling från Uppsala : Acta Universitatis Upsaliensis

    Författare :Shaman Muradrasoli; Uppsala universitet.; [2008]
    Nyckelord :Virology; Diagnosis of Viral RNA; QPCR; RNA virus; broadly targeted PCR; probe design strategy; Infectious diseases; Medicin; Medicine;

    Sammanfattning : As the area of nucleic acid based technologies develops, so will our understanding of how structural variations in DNA and RNA pathogens are associated with disease. The overall goal of this thesis is the development of broadly targeted measurement techniques for variable viral RNA by Real-Time PCR (here referred to as quantitative reverse transcriptase PCR, QRT-PCR). LÄS MER