Sökning: "nested PCR"

Visar resultat 1 - 5 av 32 avhandlingar innehållade orden nested PCR.

  1. 1. LYME BORRELIOSIS Detection and identification of the infecting genotype

    Författare :Katharina Ornstein; Sektion III; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Parasitologi människa och djur ; Parasitology human and animal ; Infektioner; Infections; PCR; genospecies; Lyme borreliosis; Borrelia burgdorferi; Microbiology; bacteriology; virology; mycology; Mikrobiologi; bakteriologi; virologi; mykologi;

    Sammanfattning : Lyme borreliosis is caused by the spirochete Borrelia burgdorferi sensu lato, a bacterium with a parasitic life-style, which is transmitted between hosts by the Ixodes tick-vector. Humans will incidentally become infected. The resulting illness can involve the skin, nervous system, joints and heart. LÄS MER

  3. 2. Food selection and fitness optimisation in insects

    Författare :Helene Bracht Jörgensen; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; food preference; nested PCR; fungal DNA; växtparasitologi; plant parasitology; Entomology; Entomologi; fungi; seeds; ground beetles; collembolans;

    Sammanfattning : To optimise fitness is a continuous process which assists species survival in changing environments. Choosing an optimal diet is an essential part of the optimisation process and a key element of optimal foraging theory. LÄS MER

  4. 3. Laboratory diagnosis of Bordetella infection : DNA hybridization and PCR validated against serology and culture in a phase 3 vaccine efficacy trial

    Författare :Elisabet Reizenstein; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : The present study has focused on developing and validating assays alternative to culture for detection of Bordetella pertussis. The sensitivity of culture is comparatively low when compared to serology, varying from 30% to 80%. An attempt to increase this sensitivity was made by development of assays detecting the DNA of the bacteria. LÄS MER

  5. 4. Detection and epidemiologic subtyping of Legionella pneumophilia using DNA-based molecular methods

    Författare :Sverker Bernander; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Legionella; AFLP; PFGE; Nested PCR; lag-1; hospital; outbreaks;

    Sammanfattning : Legionella pneumophila causes 2 - 6 % of hospitalised pneumonia cases. Several community- and hospital-acquired outbreaks have been reported over the years. Legionnaires' disease (LD) or legionellosis is also a frequent cause of pneumonia amongst travellers. LÄS MER

  7. 5. Detection and identification of Pneumocystis carinii-DNA : emphasis on laboratory diagnosis and occurrence in air

    Författare :Mats Olsson; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :Pneumocystis carinii; PCP; PCR; diagnosis; genotyping; air filtration.;

    Sammanfattning : Pneumocystis carinii is a fungal opportunistic pathogen, which causes serious pneumonia (P. carinii pneumonia - PCP) in patients with AIDS, leukaemia, and other immunodeficient conditions. The laboratory diagnosis is dependant on microscopic demonstration of the organism. LÄS MER