Sökning: "nanoelectrospray ionization"

Hittade 5 avhandlingar innehållade orden nanoelectrospray ionization.

  1. 1. New methods for sensitive analysis with nanoelectrospray ionization mass spectrometry

    Författare :Patrik Ek; Johan Roeraade; Åsa Emmer; Andries P. Bruins; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; mass spectrometry; electrospray ionization; nanoelectrospray ionization; adjustable gap; emitter; miniaturization; improved sensitivity; nanoliter picoliter sample volumes; Alzheimer’s disease; amyloid-beta Aβ ; Analytical chemistry; Analytisk kemi;

    Sammanfattning : In this thesis, new methods that address some current limitations in nanoelectrospray mass spectrometry (nESI-MS) analysis are presented. One of the major objectives is the potential gain in sensitivity that can be obtained when employing the proposed techniques. LÄS MER

  3. 2. Mass Spectrometry with Electrospray Ionization from an Adjustable Gap

    Författare :Patrik Ek; Åsa Emmer; Johan Roeraade; Per M. Claesson; Ulrika Nilsson; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Electrospray ionization; mass spectrometry; nanoelectrospray; ESI; MS; chip; emitter; gap; liquid bridge; nozzle; peptides; Analytical chemistry; Analytisk kemi;

    Sammanfattning : In this thesis the fabrication and analytical evaluation of two new electrospray emitters utilized for mass spectrometry analysis is presented. The emitters are based on a new concept, where the spray orifice can be varied in size. The thesis is based on two papers. All present-day nanoelectrospray emitters have fixed dimensions. LÄS MER

  4. 3. Sample preconcentration in open microchannels : Combinations with MALDI and nano-ESI mass spectrometry and computer simulations

    Författare :Saara Mikkonen; Åsa Emmer; Leopold Ilag; Jenny Ottosson Takanen; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Preconcentration; open microchannel; microchip; MALDI; electrospray ionization; nanoelectrospray; mass spectrometry; computer simulation;

    Sammanfattning : In this thesis a novel concept for preconcentration of biomolecules in open microchannels is presented. The preconcentration is based on electromigration of charged analytes, and detection is performed with matrix-assisted laser desorption/ionization (MALDI) or nano-electrospray ionization (nESI) mass spectrometry (MS). LÄS MER

  5. 4. Miniaturized Techniques for Protein Analysis

    Författare :Johan Sjödahl; KTH; []
    Nyckelord :Proteins; peptides; proteases; Antarctic krill; Euphausia superba; capillary electrophoresis; fluorosurfactants; mass spectrometry; nanoelectrospray; ESI; MALDI; chip; high-throughput; reproducibility; sensitivity; mass accuracy;

    Sammanfattning : Proteins are a highly diversified group of molecules, andfor their study, advanced analytical tools are required. Inparticular, a need for high-throughput techniques has emergedin order to enable the characterization of large sets ofproteins. LÄS MER

  7. 5. Electrospray Ionization Mass Spectrometry for Determination of Noncovalent Interactions in Drug Discovery

    Författare :Kurt Benkestock; Johan Roeraade; Renato Zenobi; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; electrospray ionization; drug discovery; noncovalent interaction; complexes; human serum albumin HSA ; fatty acid binding protein FABP ; ribonuclease; ligand screening; Analytical chemistry; Analytisk kemi;

    Sammanfattning : Noncovalent interactions are involved in many biological processes in which biomolecules bind specifically and reversibly to a partner. Often, proteins do not have a biological activity without the presence of a partner, a ligand. LÄS MER