Sökning: "multiplexed gene detection"

Visar resultat 1 - 5 av 9 avhandlingar innehållade orden multiplexed gene detection.

  1. 1. Electric DNA chips for determination of pathogenic microorganisms

    Författare :Yanling Liu; Sven-Olof Enfors; Thomas Schweder; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; electric DNA chip array; fast determination; virulence genes; multiplexed gene detection; bacterial colony; ultrasonication; DNA fragmentation; Bacillus cereus; Biology; Biologi; Bioengineering; Bioteknik;

    Sammanfattning : Silicon-based electric DNA chip arrays were utilized to fast identify pathogenic microorganisms with respect to the capacity to produce toxins involved in foodborne poisoning and infections. Bacteria of the B. cereus and the enterohemorrhagic E. LÄS MER

  3. 2. Molecular detection and characterization of drug-resistant Mycobacterium tuberculosis

    Författare :Anna Engström; Karolinska Institutet; Karolinska Institutet; []
    Nyckelord :;

    Sammanfattning : Tuberculosis (TB) is an ancient disease, but not a disease of the past. Despite the declaration of TB as a global emergency by the World Health Organization in 1993 the worldwide problem of TB has worsened. LÄS MER

  4. 3. Accessing Genetic Variation by Microarray Technology

    Författare :Katarina Lindroos; Edwin Southern; Uppsala universitet; []
    Nyckelord :Medical sciences; microarrays; SNPs; minisequencing; four-colour detection; allele frequency; population study; evolutionary biology; MEDICIN OCH VÅRD; MEDICINE; MEDICIN; Molekylär medicin; Molecular Medicine;

    Sammanfattning : Microarray technology is a promising approach for the simultaneous analysis of multiple single nucleotide polymorphisms (SNPs), which are the most abundant form of genetic variation. In this thesis enzyme-assisted microarray-based methods were developed to improve the accuracy and genotype discrimination power of the current methods for SNP genotyping. LÄS MER

  5. 4. RNA-based spatial characterization of cell and tissue heterogeneity

    Författare :Elin Lundin; Mats Nilsson; Rickard Sandberg; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Padlock probes; in situ sequencing; single cell resolution; single nucleotide variant resolution; spatial transcription profiling; Biochemistry; biokemi;

    Sammanfattning : Technical advances in cell biology have revolutionized the field of cell biology. With new technology it is now possible to address scientific questions in cell biology at the molecular level. LÄS MER

  7. 5. In situ Sequencing : Methods for spatially-resolved transcriptome analysis

    Författare :Marco Mignardi; Mats Nilsson; Xiaoliang Sunney Xie; Stockholms universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probes; rolling circle amplification; in situ sequencing; spatially-resolved transcriptomics; molecular diagnostics; Biochemistry; biokemi;

    Sammanfattning : It is well known that cells in tissues display a large heterogeneity in gene expression due to differences in cell lineage origin and variation in the local environment at different sites in the tissue, a heterogeneity that is difficult to study by analyzing bulk RNA extracts from tissue. Recently, genome-wide transcriptome analysis technologies have enabled the analysis of this variation with single-cell resolution. LÄS MER